Difference between revisions of "Suppressor Logic Toolkit and Quasi-Hash Function"
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pR [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0051 R0051]2009 Kit Plate 1 6K pSB1A2 | pR [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0051 R0051]2009 Kit Plate 1 6K pSB1A2 | ||
− | pLacIQ1 [http://partsregistry.org/wiki/index.php/Part:BBa_K091112 K091112] | + | pLacIQ1 [http://partsregistry.org/wiki/index.php/Part:BBa_K091112 K091112]MWSU |
pTetR [http://partsregistry.org/wiki/index.php/Part:BBa_R0040 R0040]2009 Kit Plate 1 6I pSB1A2 | pTetR [http://partsregistry.org/wiki/index.php/Part:BBa_R0040 R0040]2009 Kit Plate 1 6I pSB1A2 | ||
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RBS(B0030)+GFP [http://partsregistry.org/wiki/index.php/Part:BBa_E5500 E5500]2007 Parts Kit Plate 1 9J pSB1A2 | RBS(B0030)+GFP [http://partsregistry.org/wiki/index.php/Part:BBa_E5500 E5500]2007 Parts Kit Plate 1 9J pSB1A2 | ||
− | RBS( | + | RBS(B0034)+RFP MWSU |
Mnt [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0072 C0072]2009 Kit Plate 1 12L pSB2K3 | Mnt [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0072 C0072]2009 Kit Plate 1 12L pSB2K3 | ||
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pLuxT | pLuxT | ||
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+ | frame shift suppressor 1 CU'''GUCCU'''AA | ||
− | + | frame shift suppressor 2 UU'''ACUAG'''AC | |
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− | frame shift suppressor 2 | ||
== References == | == References == |
Revision as of 16:24, 21 May 2009
Contents
Suppressor Logic Toolkit and Quasi-Hash Function
The following slides describe the basic toolkit design and a specific experiment that would result in a quasi-hash function
Basic Toolkit Design
The AND gate was designed by Anderson using termination suppressors, while the new design would use frame shift suppressors.
![Picture13.gif](/GcatWiki/images/3/3a/Picture13.gif)
![Picture14.gif](/GcatWiki/images/c/c9/Picture14.gif)
![Picture15.gif](/GcatWiki/images/5/5f/Picture15.gif)
![Picture16.gif](/GcatWiki/images/3/3d/Picture16.gif)
Toolkit with Specific Inputs and Outputs
PprpB/propionate and luxO/pluxT are new communication players that would need to be looked into further to verify their usefulness.
See references at bottom of page.
![Picture17.gif](/GcatWiki/images/5/5f/Picture17.gif)
![Picture29.gif](/GcatWiki/images/9/90/Picture29.gif)
![Picture30.gif](/GcatWiki/images/f/f3/Picture30.gif)
![Picture31.gif](/GcatWiki/images/8/85/Picture31.gif)
Experimental Design
![Picture21.gif](/GcatWiki/images/a/a9/Picture21.gif)
![Picture22.gif](/GcatWiki/images/0/07/Picture22.gif)
Parts Needed
Parts from Registry
Promoters
pR R00512009 Kit Plate 1 6K pSB1A2
pLacIQ1 K091112MWSU
pTetR R00402009 Kit Plate 1 6I pSB1A2
pLuxR R00622009 Kit Plate 1 6O pSB1A2
pMnt R00732009 Kit Plate 1 16B pSB2K3
Genes
RBS+lamba cI+TT P04512009 Kit Plate 2 1N pSB1AK3
pBAD+RBS+LacI+TT S03980MWSU
pBAD+RBS+tetR+TT K091145MWSU
pBAD+RBS+luxR+TT K091179MWSU
RBS(B0030)+GFP E55002007 Parts Kit Plate 1 9J pSB1A2
RBS(B0034)+RFP MWSU
Mnt C00722009 Kit Plate 1 12L pSB2K3
LuxO-P K1310222009 Kit Plate 3 8K pSB1AC3
Parts needed to be built from scratch
Pprp sequence within the prp operon[1]
prpB 2-methylisocitrate lyase in E.coli MG1655 [2]
pLuxT
frame shift suppressor 1 CUGUCCUAA
frame shift suppressor 2 UUACUAGAC
References
Powerpoint File:Suppressorlogic3.ppt
Anderson et al. 2007. “Environmental signal integration by a modular AND gate” [3]
luxO luxT [4]
PprpB propionate [5]