Difference between revisions of "8) Start codon issues"
Karicheson (talk | contribs) (→Alternative Start Codon Issues) |
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There is not much published research I could find on this issue but the articles/links below are summarized: | There is not much published research I could find on this issue but the articles/links below are summarized: | ||
| + | Yokobori, et al establishes that ". . . potential start codons ATR (Met), ATT (Ile), TTG (Leu), and GTG (Val) . . . " do not all encode Met. I unfortunately only received references to this article through other articles, but I will continue searching for the following article (which may hold some answers). | ||
| + | Yokobori, S., Ueda, T., Feldmaier-Fuchs, G., Paabo, S., Ueshima, R., Kondow, A., Nishikawa, K., and Watanabe, K. 1999. Complete DNA sequence of the mitochondrial genome of the ascidian Halocynthia roretzi (Chordata, Urochordata). Genetics 153: 1851–1862. [PubMed] | ||
| − | + | [http://en.wikipedia.org/wiki/Start_codon Wikipedia] and other basic Encylopedia's claim the following. "Interestingly when an alternative start codon is used, it gets translated as methionine - even if the codon might normally encode a different amino acid. This is because a separate [tRNA] is used for initiation." | |
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1. [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TD0-3TDR71X-D&_user=2665120&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1006327537&_rerunOrigin=scholar.google&_acct=C000058476&_version=1&_urlVersion=0&_userid=2665120&md5=84e8a1e982ec7f0b9c7d17c9573c26c0 Transcription and translation in Archaea: a mosaic of eukaryal and bacterial features.] | 1. [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TD0-3TDR71X-D&_user=2665120&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1006327537&_rerunOrigin=scholar.google&_acct=C000058476&_version=1&_urlVersion=0&_userid=2665120&md5=84e8a1e982ec7f0b9c7d17c9573c26c0 Transcription and translation in Archaea: a mosaic of eukaryal and bacterial features.] | ||
| + | |||
| + | 2. [http://www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi The Genetic Codes] | ||
Revision as of 22:06, 9 September 2009
Alternative Start Codon Issues
Possible start codons AUG (most common in archaea), GUG and UUG [1].
Olivia came across a very interesting conundrum because she checked and tried to verify a lot of the computer's work:
When the start codon is not AUG, is the first amino acid in the protein's primary sequence a Met or the correct amino acid for that codon triplet?
I think it would be an interesting side study but it may require wet lab work to decipher whether or not the first amino acid is really Met when an alternative start codon is used. It may also be interesting to look for a trend in the genes that begin with an alternative start codon.
There is not much published research I could find on this issue but the articles/links below are summarized:
Yokobori, et al establishes that ". . . potential start codons ATR (Met), ATT (Ile), TTG (Leu), and GTG (Val) . . . " do not all encode Met. I unfortunately only received references to this article through other articles, but I will continue searching for the following article (which may hold some answers).
Yokobori, S., Ueda, T., Feldmaier-Fuchs, G., Paabo, S., Ueshima, R., Kondow, A., Nishikawa, K., and Watanabe, K. 1999. Complete DNA sequence of the mitochondrial genome of the ascidian Halocynthia roretzi (Chordata, Urochordata). Genetics 153: 1851–1862. [PubMed]
Wikipedia and other basic Encylopedia's claim the following. "Interestingly when an alternative start codon is used, it gets translated as methionine - even if the codon might normally encode a different amino acid. This is because a separate [tRNA] is used for initiation."
Good Articles to Reference:
1. Transcription and translation in Archaea: a mosaic of eukaryal and bacterial features.
