Difference between revisions of "Blasting Spacers for Our Genome"
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Revision as of 16:20, 10 November 2009
Blasting Spacers
CRISPR one results from CRISPR finder are shown below. I blasted all of the spacers using the nr/nt database and the results are shown below.
Spacer One: TGCGTCGTCCGGTGGCCGTCAATAAATGTCGCAAGGG
Matches by chance!
Spacer Two: TCCTACGACCTCGTCGGCGTCAACGGCTGGCCCGA
Spacer Three: CACCCTACAACAGGTGAAATCTACCAGACAAAAGA
Spacer Four: TCACCCAAGCGCAAGCAACAGCTGATCGAGGACCTG
Spacer Five: GCGACGGCGGCCAGTTCCGCGAGGGCGGGAAGGTCC
This image is only part of it. . .
NOTE: lots of hits from each organism- indicates that multiple parts have been incorporated into our species' spacers. . . interesting to study what genes have been inserted
Spacer Six: TGCGAGTGTTGCGGGGAACCGACTCGGGTAGGCCAG
No viral insertions in this spacer
Spacer Seven: ACGGTTTCGCTACCACCATCGCCACCAGCAACTGCCG
Spacer Eight: GACGAGTATGCCAACCGGCTCGTGAGCGGGCGC
E-values not good- no significant hits!
Spacer Nine: TGGTAGGCGTCGTAGGTGTTCGTGGCGAGCGTGTC
Spacer Ten: ACTCACTGGATTATGACCCCTACAACGAGGGCGTCA
No significant hits. . . lowest E-value is 1.5
Spacer Eleven: GGATCTCGATCGTTGTAGTATCCATAGCTGCTATACC
Spacer Twelve: GAAGTAACGCAACTCCAGTGAGCGCTACTGAGAGCCC
No significant hits. . . lowest E-value is 1.6
Spacer Thirteen:CCGATCACGCCCTGCCGATACTGGTAGTTCGCGATA
Spacer Fourteen: TCGTCGGCCGGCTCGTCGGCCGACGTGGACTTGC
Spacer Fifteen: AGTAGGTCTAATGTCTCTCTGTCGTCTATCAGCCCCG
No significant hits. . . lowest E-value is 1.6
Spacer Sixteen: GCTCTCCGGTGTCACAGGTCAGGTCACGGTCTCCGC
No significant hits . . . lowest E-value is 1.5
Spacer Seventeen: ACGGACAAGTCATCCACCCGCCAGTATCTCCCGGT
No significant hits . . . lowest E-value is 1.4
Spacer Eighteen: AAATACGATCCTGCGGTGACGCTACGTCCGGGGCAGC
No significant hits . . . lowest E-value is 6.4
Spacer Nineteen: CAGATGTGGGGTCTGTGGCCACAGTCTAACATCTCT
Spacer Twenty: CCTGATAACGGACTCTTGTAGGTCCGTTAGGTCGT
No significant hits . . . lowest E-value is 22
Spacer Twenty-One: AAAAATGAGTGACGTAGACATTCGGCAAAATGCCGG
No significant hits . . . lowest E-value is 6
Spacer Twenty-Two: CAGCAGCGAAACGAGCCGTCCGTCCTTTTGAGACA
No significant hits . . . lowest E-value is 1.2
Spacer Twenty-Three: GTCTAGCCCAGTCTGGTCGGGGTGGTCGGCAGGATCGG
No significant hits . . . lowest E-value is 0.43
Spacer Twenty-Four: CACTCCTCATATGTCTGTTCGAGCAGCGGGACGTG
No significant hits . . . lowest E-value is 1.4
Spacer Twenty-Five: ACCGTTGCCGCCGATCGGCAGCGAGCCGGTGATGTGT
Spacer Twenty-Six: TCAAAGCGAGCCTCGAACGCGACGACGAAGATATG
Spacer Twenty 27:
