Difference between revisions of "How to compare genome sequences using Katie's webpage"
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Firstly, your two sequences <i>must</i> be in FASTA format in order to use our Pairwise Genomic Comparison program. If your sequences are in GenBank format (or another format), visit Claudia's tutorial page first to learn how to convert your sequences to FASTA.<br><br> | Firstly, your two sequences <i>must</i> be in FASTA format in order to use our Pairwise Genomic Comparison program. If your sequences are in GenBank format (or another format), visit Claudia's tutorial page first to learn how to convert your sequences to FASTA.<br><br> | ||
| − | Once you have the two sequences you want to compare in FASTA format, head to the [http://www.example.com Pairwise Genomic Comparison] page. (Note: I'll add the link when Katie finalizes the webpage.) | + | Once you have the two sequences you want to compare in FASTA format, head to the [http://www.example.com Pairwise Genomic Comparison] page. (Note: I'll add the link when Katie finalizes the webpage.) Enter your desired Expect (E), or threshold value. Keep in mind that lower E values will be more restrictive and lead to less matches by chance (though any matches found will be more statistically significant). For our purposes, using an E value around 0.001 or 0.01 should be sufficient. |
Revision as of 04:29, 12 November 2009
Firstly, your two sequences must be in FASTA format in order to use our Pairwise Genomic Comparison program. If your sequences are in GenBank format (or another format), visit Claudia's tutorial page first to learn how to convert your sequences to FASTA.
Once you have the two sequences you want to compare in FASTA format, head to the Pairwise Genomic Comparison page. (Note: I'll add the link when Katie finalizes the webpage.) Enter your desired Expect (E), or threshold value. Keep in mind that lower E values will be more restrictive and lead to less matches by chance (though any matches found will be more statistically significant). For our purposes, using an E value around 0.001 or 0.01 should be sufficient.
