Difference between revisions of "Disease resistance to fungal diseases"

Latest revision as of 23:39, 15 April 2013

Media:Potential_Genes_of_Interest.xlsx

Background on Resistance (R) genes and avirulence (Avr) genes

(Hammond-Kosack & Jones 1997)

R genes are found in the plant, while corresponding Avr gene is in the pathogen

Presumed roles of R genes:

1. help plants detect pathogenic Avr gene products
2. initiate signal transduction pathways that will help defend against the pathogen
3. "have the capacity to evolve new R gene specificities rapidly"

If the R gene or corresponding pathogenic Avr gene are not present or altered, then plant is infected with the disease

The R protein recognizes the Avr gene product (ligand), activating a signal transduction cascade that initiates the defense against the pathogen. Thus, the R gene is turned on in healthy plants. R proteins must be able to evolve quickly in order to target new pathogens specifically. The evolution of the Avr genes directly impacts the evolution of the related R gene (coevolution).

(Parker 2003)

"The majority of characterized plant R proteins resemble modular structures of the LRR-containing Toll-like receptors mediating animal innate immunity or the more recently discovered intracellular nucleotide-binding domain-LRR (Nod) proteins that are also implicated in PAMP perception in humans (Fig. 1) [4,13]. It is therefore reasonable to speculate that R-protein-mediated recognition in plants shares mechanistic features of non-self discrimination processes in animal cells."

3 Common Blueberry Fruit Rots

[ohioline.osu.edu/hyg-fact/3000/pdf/3213.pdf OSU]

1. Alternaria - postharvest rot; most common

Caused by Alternaria tenuissima

1. Anthracnose - serious pre- and postharvest disease

Caused by Colletotrichum acutatum (Yoshida et al. 2007)

1. Botrytis - normally minor, but can be severe

Caused by Botrytis cinerea (genomic analysis of Botrytis, a necrotrophic fungus)

General Information & Genes

Parker 2003
-"innate immunity" through general elicitors (basically, molecules that are not specific to a certain cultivar)

Feys & Parker 2000

Explanation of Chitin Signaling and Fungal Invasion Response

-plants can recognize “surface-derived molecules” that elicit a general immune response (this is in addition to R gene-mediated pathways)
–pathogen-associated or microbe-associated molecular patterns (PAMPs/MAMPs)
-PAMPs are important for microbial invasion of a host cell
-glucans, chitins, and proteins derived from fungal cell walls serve as elictors, or “pathogen derived molecules” (Montesano et al 2003)
-during fungal infection, chitinases in the infected cell degrade the chitin on the cell wall of the invading fungus
-the remaining chitin fragments (chitooligosaccarides) then also serve as elicitors that provoke necessary “defense response genes”
-LysM RLKs (LsyM domain-containing receptor-like kinases): relatively large plant-specific protein family that researchers may suggest play a significant role in detecting fungal chitin

Ceasar & Ignacimuthu 2012 identified genes (mainly chitinases and glucanases) used into genetic engineering of crop plants. The tables below list important list the genes transformed and their source organism.

Alternaria

Feys & Parker 2000 outlined the signalling cascade for Alternaria in Arabidopsis:

Jia et al. 2013 discusses the role of 3 plant hormones (ethlynene [ET], jasmonic acid [JA], and salicylic acid [SA]) in necrotrophic fungal infections (such as Alternaria alternata) in the tomato. They ultimately suggest the following pathway:

Thomma et al 1998 elucidated some of the genes in the JA and SA pathways in Arabidopsis. PR-1, PR-2 (a B-1,3-glucanase), and PR-5 follow an SA-dependent pathway.

PDF1.2 (a common plant defensin), PR-3 (a chitinase), and PR-4 (a hevein-like protein) depend on the JA pathway

Colletotrichum

Miles et al. 2011 identified ESTs in a Colletotrichum-resistant blueberry strains that were highly homologous to known defense genes, including a class II chitinase, pathogenesis-related protein 10 (PR10), and β-1,3-glucanase. They also found ESTs, such as monodehydroascorbate reduc- tase (EST03) and a metallothionein-like protein (EST05), associated with oxidative stress, which helps protect the plant from oxidative damage.

Gene-to-Gene Resistance

Glazebrook 2001

"Mutations in PBS1, NDR1, EDS1, PAD4, and PBS2 block gene-for-gene resistance that is mediated by some R genes." 3 different signal transduction cascades acting downstream of R genes: 1) NDR-1 (and PBS-2) dependent, 2) EDS1 (and PAD4) dependent, 3) not sure of dependence

Glazebrook 2005

"According to the model, gene-for-gene resistance should not be observed in in- teractions with necrotrophs, as host cell death is not predicted to limit pathogen growth. Furthermore, SA-dependent responses and SAR are not predicted to play a role, whereas responses mediated by JA and ET are expected to play a role."

-includes interesting take on biotrophic vs. necrotrophic fungi on Arabidopsis that contradicts what the plant guy said about SAR/ISR pathways

Patent on "Plant Genes Involved in Defense Against Pathogens"

-explanation of R gene signal transduction, SA-dependent signaling, JA-dependent signaling, and induced systemic resistance (ISR) -particularly, explains the signal transduction pathways for EDS1 and NDR1

Plant NBS-LRR Resistance Gene Homologues

-Fig. 1 lists some NBS-LRR genes important in plant resistance

DeYoung & Innes 2006

-explanation of Plant NBS-LRR proteins

Things to Look Up

Abscisic acid (AB) interactions with JA and ET pathways

KEGG Pathway - environmental information processing - signal transduction - plant hormone transduction (look at dormancy)

Differential expression of strawberry genes as a result of Colletotrichum expression