Difference between revisions of "MWSU Lab"
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(Created page with " == To Do == 1) Get melamine deaminase in correct location in the plasmid. 2) Retest riboswitches with the biosensor. 3) Test riboswitches in ThyA fitness module (insert ThyA-...") |
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== To Do == | == To Do == | ||
1) Get melamine deaminase in correct location in the plasmid. | 1) Get melamine deaminase in correct location in the plasmid. | ||
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2) Retest riboswitches with the biosensor. | 2) Retest riboswitches with the biosensor. | ||
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3) Test riboswitches in ThyA fitness module (insert ThyA- into plasmid). | 3) Test riboswitches in ThyA fitness module (insert ThyA- into plasmid). | ||
| − | 4) Find a way to better dissolve ammeline (e.g. instead of toxic NaOH, use DMSO) | + | |
| + | 4) Find a way to better dissolve ammeline (e.g. instead of toxic NaOH, use DMSO). | ||
| + | |||
5) Research for more riboswitch options. | 5) Research for more riboswitch options. | ||
| + | |||
6) Use UNAFold and futher study of Dixon article to create new riboswitches. | 6) Use UNAFold and futher study of Dixon article to create new riboswitches. | ||
| + | |||
7) Use pLac promoter instead of P5. | 7) Use pLac promoter instead of P5. | ||
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8) Put it all together: melamine deaminase, riboswitch, etc. This allows for the testing of different promoters and ribosome binding sites to optimize the fitness module. | 8) Put it all together: melamine deaminase, riboswitch, etc. This allows for the testing of different promoters and ribosome binding sites to optimize the fitness module. | ||
Revision as of 18:38, 21 May 2014
To Do
1) Get melamine deaminase in correct location in the plasmid.
2) Retest riboswitches with the biosensor.
3) Test riboswitches in ThyA fitness module (insert ThyA- into plasmid).
4) Find a way to better dissolve ammeline (e.g. instead of toxic NaOH, use DMSO).
5) Research for more riboswitch options.
6) Use UNAFold and futher study of Dixon article to create new riboswitches.
7) Use pLac promoter instead of P5.
8) Put it all together: melamine deaminase, riboswitch, etc. This allows for the testing of different promoters and ribosome binding sites to optimize the fitness module.
