Difference between revisions of "May 28, 2014"
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I conducted a serial dilution of NaOH in water to see which NaOH concentration 100 mM Amm could dissolve in. After heating and vortexing, 100 mM Amm was able to dissolve in 0.125 N NaOH. However, 125 mM Amm was unable to dissolve in 0.125 N NaOH. I am using 0.125 N HCl to neutralize the NaOH in order to make the solution less toxic for ''E. Coli'' cells. | I conducted a serial dilution of NaOH in water to see which NaOH concentration 100 mM Amm could dissolve in. After heating and vortexing, 100 mM Amm was able to dissolve in 0.125 N NaOH. However, 125 mM Amm was unable to dissolve in 0.125 N NaOH. I am using 0.125 N HCl to neutralize the NaOH in order to make the solution less toxic for ''E. Coli'' cells. | ||
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| + | After mini-prepping TriA in pMK and RFP in pSB1A2-BR, the DNA was digested with X and S to isolate pSB1A2-BR and TriA. | ||
Latest revision as of 21:46, 28 May 2014
I tried to dissolve 5 mM Amm in 4 mL DMSO using a 55 C water bath without success. DMSO might dissolve in warmer temperatures.
I conducted a serial dilution of NaOH in water to see which NaOH concentration 100 mM Amm could dissolve in. After heating and vortexing, 100 mM Amm was able to dissolve in 0.125 N NaOH. However, 125 mM Amm was unable to dissolve in 0.125 N NaOH. I am using 0.125 N HCl to neutralize the NaOH in order to make the solution less toxic for E. Coli cells.
After mini-prepping TriA in pMK and RFP in pSB1A2-BR, the DNA was digested with X and S to isolate pSB1A2-BR and TriA.
