Difference between revisions of "Repeating Eckdahl's 20-clone selection"
m (Jolim moved page Repeating 20 Clone Experiments to Repeating Eckdahl's 20-clone selection) |
|||
| Line 6: | Line 6: | ||
Protocol created on 05/21/14: [[Eckdahl-replication-protocol]] | Protocol created on 05/21/14: [[Eckdahl-replication-protocol]] | ||
| + | |||
| + | [https://www.dropbox.com/sh/2jqkqfz755ng0vc/AAA26M9kEeYii0pFtFlCQ17na Photos from Davidson's first replication (four plates)] | ||
Revision as of 19:28, 29 May 2014
We need to replicate Dr. Eckdahl’s 20-clone experiment twice on both campuses and mathematically analyze the data. Continue adding arabinose to induce the chaperones.
We need to grow the surviving clones on individual plates: no antibiotic, amp, amp+chlor. This will enable us to determine whether both plasmids (CDM+amp, chaperone+chlor) are still present and being transcribed.
Depending on the survivorship of the clones in the previous step, set up plates to run head-to-head competition between two surviving clones, and determine which strain is most competent. This will confirm the results of the 20-clone experiment, in a more controlled environment than a plate with all 20 clones.
Protocol created on 05/21/14: Eckdahl-replication-protocol
