Difference between revisions of "NHE Feb 2 Notes"
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**normalizes for the length of the gene per million reads | **normalizes for the length of the gene per million reads | ||
**FPKM values | **FPKM values | ||
| + | |||
| + | Used R and DESeq data to generate heat maps of differentially expressed genes in both liver and intestine | ||
| + | Also tired to separate fed and fasted within the liver and intestine groups. (Modified R code) | ||
| + | |||
| + | *noticed that one of the fed (animal 4?) was being grouped as more similar to the fasted animals than the other fed. | ||
Revision as of 18:46, 4 February 2016
BLAST Results:
- most were mitochondrial
We can download the sequences from the 2015 paper, but not the list of genes 2013 Paper has list of genes with gene ontology
We are using DESeq standard to normalize(?) data
- used to normalize expression
- normalizes for the length of the gene per million reads
- FPKM values
Used R and DESeq data to generate heat maps of differentially expressed genes in both liver and intestine Also tired to separate fed and fasted within the liver and intestine groups. (Modified R code)
- noticed that one of the fed (animal 4?) was being grouped as more similar to the fasted animals than the other fed.
