Difference between revisions of "Gel Electrophoresis for Bio113"
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# You will start with the tubes containing your PCR experiments. You should have some eXperimental tubes and one Negative control tube. | # You will start with the tubes containing your PCR experiments. You should have some eXperimental tubes and one Negative control tube. | ||
# Take 20 µL of each green PCR solution and load each into its own well. Record the location of each sample you load. | # Take 20 µL of each green PCR solution and load each into its own well. Record the location of each sample you load. | ||
| − | # Dr. C. will load 5 µL of the 1 kb molecular weight marker ([http://www.bio.davidson.edu/people/macampbell/113/weekly_Labs/1kbladder.png also called 1 kb ladder). | + | # Dr. C. will load 5 µL of the 1 kb molecular weight marker ([http://www.bio.davidson.edu/people/macampbell/113/weekly_Labs/1kbladder.png also called 1 kb ladder]). |
# The gel contains Midori Green which is a dye that fluoresces green when it is bound to dsDNA. | # The gel contains Midori Green which is a dye that fluoresces green when it is bound to dsDNA. | ||
# The gel will run at about 100 volts for about 30 minutes. We will photograph the gel and you will get your own hard copy of the final results. | # The gel will run at about 100 volts for about 30 minutes. We will photograph the gel and you will get your own hard copy of the final results. | ||
Latest revision as of 15:49, 19 July 2017
Analyzing PCR Results Using Gel Electrophoresis
- A 2.0% agarose gel has been poured for you. It contains 0.5X TBE buffer.
- You will start with the tubes containing your PCR experiments. You should have some eXperimental tubes and one Negative control tube.
- Take 20 µL of each green PCR solution and load each into its own well. Record the location of each sample you load.
- Dr. C. will load 5 µL of the 1 kb molecular weight marker (also called 1 kb ladder).
- The gel contains Midori Green which is a dye that fluoresces green when it is bound to dsDNA.
- The gel will run at about 100 volts for about 30 minutes. We will photograph the gel and you will get your own hard copy of the final results.
