Difference between revisions of "Davidson Missouri W/Davidson Protocols"

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m (The What's and How's moved to Davidson Missouri W/Davidson Protocols)
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* What is the list of DNA parts that we will need for the first stage, second stage, entire project?<br>
 
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*<u>ERIN and SABRIYA</u> Which DNA parts exist in the registry?[[Answer1]][[More Parts]]<br>
 
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* <u>ERIN and SABRIYA</u>Which DNA parts will have to be designed?  Will they be synthesized or produced by PCR? <br>
 
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[[loxP sites]] [[Hin/Hix]] [[CRE gene]] [[Degradation Tags]]
 
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# [http://www.bio.davidson.edu/courses/Molbio/Protocols/reagents.html Common molecular reagents]
 
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/reagents.html Common molecular reagents]
 
# [http://parts.mit.edu/registry/index.php/Assembly:Standard_assembly Standard Assembly]
 
# [http://parts.mit.edu/registry/index.php/Assembly:Standard_assembly Standard Assembly]

Revision as of 20:00, 11 July 2007

  1. Common molecular reagents
  2. Standard Assembly
  3. Compatibility of Plasmids
  4. Building dsDNA with Oligos
  5. Setting up PCR mixtures
  6. PCR and Mg2+ concentration
  7. Clean and Concentrate DNA (after PCR, before digestion)
  8. Pouring an agarose gel
  9. Calculate MWs
  10. Digest DNA with restriction enzymes
  11. Double Digest Guide
  12. Ethanol Precipitate DNA (short protocol)
  13. 1kb MW markers
  14. Shrimp Alkaline Phosphatase
  15. Qiagen QIAquick Gel Purification
  16. Qiagen QIAquick Column Regeneration Protocol
  17. ElectroElute Gel Purification
  18. Ligation Protocol
  19. Heat Shock Transformation OR Short version of Heat Shock
  20. Zippy Transformation
  21. Colony PCR to Screen for Successful Ligations
  22. Promega miniprep
  23. Choices for Transformation: Heat Shock vs. Zyppy
  24. Choices for Mini-Preps: Promega vs. Zyppy
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