Difference between revisions of "Tuning genetic control through promoter engineering"
From GcatWiki
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[http://www.bio.umass.edu/mcbfacs/intro.htm Helpful Flow Cytometry Link] | [http://www.bio.umass.edu/mcbfacs/intro.htm Helpful Flow Cytometry Link] | ||
| − | RT-PCR | + | '''RT-PCR''' |
| + | In molecular biology, reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique for amplifying a defined piece of a ribonucleic acid (RNA) molecule. The RNA strand is first reverse transcribed into its DNA complement or complementary DNA, followed by amplification of the resulting DNA using polymerase chain reaction. | ||
| + | |||
| + | [http://www.bio.davidson.edu/Courses/immunology/Flash/RT_PCR.html Davidson RT-PCR Animation] | ||
Revision as of 01:15, 22 September 2007
Methods
Flow Cytometry
Flow Cytometry is a technique for counting, examining, and sorting microscopic particles suspended in a stream of fluid. It allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of single cells flowing through an optical and/or electronic detection apparatus. (from Wikipedia)
RT-PCR In molecular biology, reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique for amplifying a defined piece of a ribonucleic acid (RNA) molecule. The RNA strand is first reverse transcribed into its DNA complement or complementary DNA, followed by amplification of the resulting DNA using polymerase chain reaction.
