Difference between revisions of "Rational design of memory in eukaryotic cells"
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[http://en.wikipedia.org/wiki/Fluorescence_microscopy Fluorescence Microscopy] | [http://en.wikipedia.org/wiki/Fluorescence_microscopy Fluorescence Microscopy] | ||
| − | [http://en.wikipedia.org/wiki/Differential_interference_contrast_microscopy DIC (Differential Interference Contrast Microscopy | + | [http://en.wikipedia.org/wiki/Differential_interference_contrast_microscopy DIC (Differential Interference Contrast) Microscopy] |
==Supplementary Material== | ==Supplementary Material== | ||
Latest revision as of 14:26, 9 October 2007
Paper
View pdf - Rational design of memory in eukaryotic cells Caroline M. Ajo-Franklin, et al. 2007.
Authors
Caroline Ajo-Franklin
David Drubin
Julian Eskin
Elain Gee
Dirk Landgraf
Ira Phillips
Pamela Silver
Methods
DIC (Differential Interference Contrast) Microscopy
Supplementary Material
Helpful Information
Keep in mind that the first half of the paper discusses the creation of an activator. This construct is intended to produce both red and yellow fluorescence in the presence of galactose. The second part of the paper discusses how this activator is then modified to serve as a sythetic memory device with three separate states:
Yeast that have never been in the presence of galactose will not fluoresce.
Yeast that are currently in the presence of galactose will fluoresce red (and yellow).
Yeast that have at one time been in the presence of galactose will fluoresce yellow.
Note, again, that in this paper, the creation of a bistable system relies on cooperative binding [1] (in this case, cooperative binding of an activator). Pay attention to this when looking over the math modeling.
