Difference between revisions of "Modeling Promoter Activity"
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In this 1997 paper, Jensen and Hammer constructed a library of synthetic promoters based on the ''Lactococcus lactis'' prokaryotic promoter in order to better determine how gene sequence of promoters was tied to the promoter strength. Specifically, Jensen and Hammer were looking for a way to construct a constitutively active promoter – one that was always turned on, without needing an inducer – that could be safely used to tune gene expression in industrial-scale metabolic engineering projects, where inducers might be impractical or hazardous. | In this 1997 paper, Jensen and Hammer constructed a library of synthetic promoters based on the ''Lactococcus lactis'' prokaryotic promoter in order to better determine how gene sequence of promoters was tied to the promoter strength. Specifically, Jensen and Hammer were looking for a way to construct a constitutively active promoter – one that was always turned on, without needing an inducer – that could be safely used to tune gene expression in industrial-scale metabolic engineering projects, where inducers might be impractical or hazardous. | ||
| − | In order to tune the steady-state ''L. lactis'' promoter without using an inducer, Jensen and Hammer had to create a library of ''L. lactis'' mutant promoters, all with various levels of activity. To generate the library, they used the method described in [[Promoters and Reporters in Synthetic Biology]]: constructing oligonucleiotides that matched the genes common to all previous ''L. lactis'' promoters and mutants. | + | In order to tune the steady-state ''L. lactis'' promoter without using an inducer, Jensen and Hammer had to create a library of ''L. lactis'' mutant promoters, all with various levels of activity. To generate the library, they used the method described in [[Promoters and Reporters in Synthetic Biology]]: constructing oligonucleiotides that matched the genes common to all previous ''L. lactis'' promoters and mutants, then allowing the oligonucleiotides to be joined together by random spacer sequences. |
=== Jensen, Alper, Fischer, and Stephanopoulis (2006): Statistical Modeling and Critical Mutation Sites === | === Jensen, Alper, Fischer, and Stephanopoulis (2006): Statistical Modeling and Critical Mutation Sites === | ||
Revision as of 20:27, 6 December 2007
Contents
Modeling Promoter Activity
In order to use synthetic promoters to their fullest potential, we have to understand how they work. Sythetic promoters cannot help us model gene circuit activity unless models are developed for the activity of the promoter itself. Determining how exactly a promoter's strength correlates to its mutations is not easy, since for the most part it requires working with promoters on the level of individual sets of nucleiotides.
Jensen and Hammer (1997): Spacer Sequences
In this 1997 paper, Jensen and Hammer constructed a library of synthetic promoters based on the Lactococcus lactis prokaryotic promoter in order to better determine how gene sequence of promoters was tied to the promoter strength. Specifically, Jensen and Hammer were looking for a way to construct a constitutively active promoter – one that was always turned on, without needing an inducer – that could be safely used to tune gene expression in industrial-scale metabolic engineering projects, where inducers might be impractical or hazardous.
In order to tune the steady-state L. lactis promoter without using an inducer, Jensen and Hammer had to create a library of L. lactis mutant promoters, all with various levels of activity. To generate the library, they used the method described in Promoters and Reporters in Synthetic Biology: constructing oligonucleiotides that matched the genes common to all previous L. lactis promoters and mutants, then allowing the oligonucleiotides to be joined together by random spacer sequences.
