Difference between revisions of "Davidson Missouri W/Davidson Protocols"
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#[http://www.bio.davidson.edu/courses/Molbio/Protocols/MiniPrep_list.html Choices for Mini-Preps: Promega vs. Zyppy] | #[http://www.bio.davidson.edu/courses/Molbio/Protocols/MiniPrep_list.html Choices for Mini-Preps: Promega vs. Zyppy] | ||
# [[Davidson Missouri W/CUGI_Seuqencing| Sequencing at CUGI]] | # [[Davidson Missouri W/CUGI_Seuqencing| Sequencing at CUGI]] | ||
| + | # [[Davidson Missouri W/Primer_dimer| Making dsDNA Using Primer Dimers]] | ||
Revision as of 19:17, 15 April 2008
- Common molecular reagents
- Standard Assembly
- Compatibility of Plasmids
- Building dsDNA with Oligos
- Setting up PCR mixtures
- PCR and Mg2+ concentration
- Clean and Concentrate DNA (after PCR, before digestion)
- Pouring an agarose gel
- Calculate MWs
- Digest DNA with restriction enzymes
- Double Digest Guide
- Ethanol Precipitate DNA (short protocol)
- 1kb MW markers
- Shrimp Alkaline Phosphatase
- Qiagen QIAquick Gel Purification
- Qiagen QIAquick Column Regeneration Protocol
- ElectroElute Gel Purification
- Ligation Protocol
- Heat Shock Transformation OR Short version of Heat Shock
- Zippy Transformation
- Colony PCR to Screen for Successful Ligations
- Promega miniprep
- Choices for Transformation: Heat Shock vs. Zyppy
- Choices for Mini-Preps: Promega vs. Zyppy
- Sequencing at CUGI
- Making dsDNA Using Primer Dimers
