Difference between revisions of "Divide and Conquer Biological Challenges"
From GcatWiki
MaCampbell (talk | contribs) (→* We need to have an XOR logic gate produced.) |
MaCampbell (talk | contribs) (→* We need to have an XOR logic gate produced.) |
||
| Line 22: | Line 22: | ||
# [http://partsregistry.org/Part:BBa_I732205 I732205 (NOT/ Dual-repressed NOR)] | # [http://partsregistry.org/Part:BBa_I732205 I732205 (NOT/ Dual-repressed NOR)] | ||
*AND: <br> | *AND: <br> | ||
| − | [[Image:AND_Gate.png]] | + | '''Existing But Complex''' |
| + | [[Image:AND_Gate.png]]<br> | ||
| + | <br> | ||
| + | '''Simple But Hypothetical''' | ||
| + | [[Image:Tet_AND_Lac.jpg]] | ||
| + | [[Image:AND_Table.jpg]] | ||
| + | |||
*NAND:<br> | *NAND:<br> | ||
# [http://partsregistry.org/Part:BBa_I732914 I732914] | # [http://partsregistry.org/Part:BBa_I732914 I732914] | ||
Revision as of 15:08, 29 May 2008
Contents
- 1 * We need to get the Lux/AHL system working.
- 2 * We need to get the Lsr/AI-2 system working.
- 3 * We do NOT need to use additional chemical input signals (e.g., aTc and IPTG). We will use only AHL and AI-2 added exogenously.
- 4 * We need to have an XOR logic gate produced.
- 5 * We need to figure out how to get cells to communicate in a sequence and not stop growing too soon.
* We need to get the Lux/AHL system working.
* We need to get the Lsr/AI-2 system working.
Our summary of the Lsr system: Davidson/Missouri_Western_iGEM2008#Lsr_.28AI-2.29_cell_signaling_system
- There are NO Lsr parts in the registry:
- We will need to make BB parts for:
- LsrR and LsrK which are adjacent to each other in the E. coli genome. We could amplify them together with a total size of over 2600bp.
- Lsr promoter
- LuxS that produces DPD that somehow is converted to R-THMF.
- How do we get cells to make AI-2?
* We do NOT need to use additional chemical input signals (e.g., aTc and IPTG). We will use only AHL and AI-2 added exogenously.
* We need to have an XOR logic gate produced.
Existing Logic Gates
- NOT:
- AND:
Existing But Complex
Simple But Hypothetical
- NAND:
- NOR:
- Inverters:
Self-Contained Inverters
PoPS Output Inverters
* We need to figure out how to get cells to communicate in a sequence and not stop growing too soon.
- What if AmpR is secreted and cells are not AmpR? This would prevent cells down the chain from responding too soon.
