Difference between revisions of "Davidson Missouri W/Davidson Protocols"
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#[http://www.bio.davidson.edu/courses/Molbio/Protocols/Tranformation_list.html Choices for Transformation: Heat Shock vs. Zyppy] | #[http://www.bio.davidson.edu/courses/Molbio/Protocols/Tranformation_list.html Choices for Transformation: Heat Shock vs. Zyppy] | ||
#[http://www.bio.davidson.edu/courses/Molbio/Protocols/MiniPrep_list.html Choices for Mini-Preps: Promega vs. Zyppy] | #[http://www.bio.davidson.edu/courses/Molbio/Protocols/MiniPrep_list.html Choices for Mini-Preps: Promega vs. Zyppy] | ||
| − | |||
# [[Davidson Missouri W/Primer_dimer| Making dsDNA Using Primer Dimers]] | # [[Davidson Missouri W/Primer_dimer| Making dsDNA Using Primer Dimers]] | ||
| + | |||
| + | '''Web Pages We Use''' | ||
| + | #[http://gcat.davidson.edu/StudentProjects/mwk/gelwebsite.html Optimize your Gel] | ||
#[http://gcat.davidson.edu/iGEM07/genesplitter.html Gene Splitting Web Site] | #[http://gcat.davidson.edu/iGEM07/genesplitter.html Gene Splitting Web Site] | ||
#[http://gcat.davidson.edu/StudentProjects/womp/testwebsite.html PCR Primers w/ BioBricks] | #[http://gcat.davidson.edu/StudentProjects/womp/testwebsite.html PCR Primers w/ BioBricks] | ||
# [http://www.promega.com/biomath/calc11.htm Promega T<sub>m</sub> Calculator] | # [http://www.promega.com/biomath/calc11.htm Promega T<sub>m</sub> Calculator] | ||
# [http://partsregistry.org/AHL List of auto-inducers and their Sigma catalog numbers] | # [http://partsregistry.org/AHL List of auto-inducers and their Sigma catalog numbers] | ||
| − | #[http://gcat.davidson.edu/ | + | # [Davidson Missouri W/CUGI_Seuqencing| Sequencing at CUGI] |
| + | #[http://gcat.davidson.edu/IGEM06/oligo.html Lance-olator Oligos for dsDNA assembly] | ||
Revision as of 15:12, 2 July 2008
- How to Keep a Lab Notebook
- Common molecular reagents
- Standard Assembly
- BioBrick Ends
- Compatibility of Plasmids
- Building dsDNA with Oligos
- Setting up PCR mixtures
- PCR and Mg2+ concentration
- Clean and Concentrate DNA (after PCR, before digestion)
- Pouring an agarose gel
- Calculate MWs
- Digest DNA with restriction enzymes
- Double Digest Guide
- Ethanol Precipitate DNA (short protocol)
- 1kb MW markers
- Shrimp Alkaline Phosphatase
- Qiagen QIAquick Gel Purification
- Qiagen QIAquick Column Regeneration Protocol
- ElectroElute Gel Purification
- Ligation Protocol
- Heat Shock Transformation OR Short version of Heat Shock
- Zippy Transformation
- Colony PCR to Screen for Successful Ligations
- Promega miniprep
- Choices for Transformation: Heat Shock vs. Zyppy
- Choices for Mini-Preps: Promega vs. Zyppy
- Making dsDNA Using Primer Dimers
Web Pages We Use
- Optimize your Gel
- Gene Splitting Web Site
- PCR Primers w/ BioBricks
- Promega Tm Calculator
- List of auto-inducers and their Sigma catalog numbers
- [Davidson Missouri W/CUGI_Seuqencing| Sequencing at CUGI]
- Lance-olator Oligos for dsDNA assembly
