Difference between revisions of "Davidson Missouri W/CUGI Sequencing"
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The sequencing account's password at Clemson is the same as the name of the computer next to the microarray scanner. The website is [http://www.genome.clemson.edu www.genome.clemson.edu] | The sequencing account's password at Clemson is the same as the name of the computer next to the microarray scanner. The website is [http://www.genome.clemson.edu www.genome.clemson.edu] | ||
| − | *You need to send at least 30 ng of DNA in a tube for each sequencing reaction. | + | *You need to send at least 30 ng of DNA in a tube for each sequencing reaction. Unless you have a good reason not to, always submit 90 ng for each primer used for sequencing. |
*The reaction volumes are 5 µl, therefore, the DNA has to be dry or in a small volume. | *The reaction volumes are 5 µl, therefore, the DNA has to be dry or in a small volume. | ||
| − | *The DNA to be sequenced must be sent dried down, or within 3 µl. | + | *The DNA to be sequenced must be sent dried down, or within 3 µl. Unless you have a good reason not to, always submit the DNA dried down. |
| − | *Primers are at 1 pmol/reaction | + | *Primers are at 1 pmol/reaction. Unless you have a good reason not to, always submit 3 pmols for each reaction you want performed. |
*$3.00 / sample | *$3.00 / sample | ||
Note by ATM: | Note by ATM: | ||
I sent 80 ng of DNA for 3 samples and 10 pmol total of primer for 3 reactions. | I sent 80 ng of DNA for 3 samples and 10 pmol total of primer for 3 reactions. | ||
Revision as of 17:28, 3 March 2009
For Dr. Campbell's Lab
The sequencing account's password at Clemson is the same as the name of the computer next to the microarray scanner. The website is www.genome.clemson.edu
- You need to send at least 30 ng of DNA in a tube for each sequencing reaction. Unless you have a good reason not to, always submit 90 ng for each primer used for sequencing.
- The reaction volumes are 5 µl, therefore, the DNA has to be dry or in a small volume.
- The DNA to be sequenced must be sent dried down, or within 3 µl. Unless you have a good reason not to, always submit the DNA dried down.
- Primers are at 1 pmol/reaction. Unless you have a good reason not to, always submit 3 pmols for each reaction you want performed.
- $3.00 / sample
Note by ATM: I sent 80 ng of DNA for 3 samples and 10 pmol total of primer for 3 reactions.
