Difference between revisions of "Protein of unknown function ("

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=Conserved hypothetical protein TIGR00162 (ID 644030738) [http://img.jgi.doe.gov/cgi-bin/geba/main.cgi?section=GeneDetail&page=geneDetail&gene_oid=644030738 644030738]=
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=Conserved hypothetical protein TIGR00162 [http://img.jgi.doe.gov/cgi-bin/geba/main.cgi?section=GeneDetail&page=geneDetail&gene_oid=644030738 644030738]=
  
=Blastn Search=
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=Blast=
Megablast and blastn gave no results other than the source genome (''Halomicrobium mukohataei''). When I ran the blast searching for more dissimilar (discontiguous megablast), the results were not helpful - they were either complete genomes or not statistically significant.
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Megablast confirmed that the sequence is 100% identical to one in (''Halomicrobium mukohataei''). Blast also showed that the microbe (''Halorhabdus utahensis'') has a sequence that is 100% identical to this particular gene, and that (''Saccharopolyspora erythraea'') has two identified genes with sequences similar to those found within this sequence (shown below).
 
 
=Blastx Search=
 
This search provided a number of results, however many were hypothetical proteins. A number of results were cupin conserved barrel domain proteins, however I was unable to determine if any of these results were relevant.
 
  
 
=GC Content=
 
=GC Content=
 
The GC content for this gene is 70% and the GC content for our species' genome is 66%. These percentages are fairly similar, thus ruling out the possibility the possibility that this is an outside gene. There may however exist paralogs and orthologs to this gene.
 
The GC content for this gene is 70% and the GC content for our species' genome is 66%. These percentages are fairly similar, thus ruling out the possibility the possibility that this is an outside gene. There may however exist paralogs and orthologs to this gene.

Revision as of 12:28, 8 September 2009

Conserved hypothetical protein TIGR00162 644030738

Blast

Megablast confirmed that the sequence is 100% identical to one in (Halomicrobium mukohataei). Blast also showed that the microbe (Halorhabdus utahensis) has a sequence that is 100% identical to this particular gene, and that (Saccharopolyspora erythraea) has two identified genes with sequences similar to those found within this sequence (shown below).

GC Content

The GC content for this gene is 70% and the GC content for our species' genome is 66%. These percentages are fairly similar, thus ruling out the possibility the possibility that this is an outside gene. There may however exist paralogs and orthologs to this gene.