CRISPR-associated Proteins

RAST and JGI agree that our genome has 6 CRISPR-associated (cas) proteins:

1) Cas1

2) Cas2

3) Cas3(helicase)

4) Cas4a (RecB family exonuclease)

5) TM1800 family

6) TM1801 family

After comparing the conserved proteins between our genome and the other 9 genomes, I found these cas-proteins were conserved:

H. sinaiiensis shares all 6 cas-proteins with H. mukohataei.

H. vallismortis shares all cas-proteins except for Cas4.

H. californiae shares all cas-proteins except for Cas4.

H. denitrificans shares all 6 cas-proteins with H. mukohataei.

H. mediteranei shares all 6 cas-proteins with H. mukohataei.

H. volcanii shares only Cas2, Cas3 and Cas4 with H. mukohataei.

H. sulfurifontis shares only Cas1, Cas3 and TM1800.

H. mucosum shares all 6 cas-proteins with H. mukohataei.

H. utahensis shares all 6 cas-proteins with H. mukohataei.

Research:

Several papers agree that Cas1 in particular is highly conserved across genomes. Only H. volcanii does not share the Cas1 gene with the other 9 genomes.