Common Restriction Sites
From GcatWiki
These restriction sites are all optimal at 37 degrees Celsius and are organized by the kind of overhang they create. *denotes it is affected by methylation (CpG, dcm, or dam)
3’ Overhang:
2 Sticky Ends-
- AsiSI *
- PacI
- PvuI-HF *
- SacII *
4 Sticky Ends-
- AatII *
- BmtI/BmtI-HF
- FseI *
- KpnI-HF
- NsiI-HF
- PstI-HF
- SacI-HF *
- SbfI/SbfI-HF
- SphI-HF
5’ Overhang:
2 Sticky Ends-
- AcII: *
- ClaI *
- NarI *
- NdeI
4 Sticky Ends-
- AflII
- AgeI-HF
- ApaLI *
- AscI *
- AvrII
- BamHI-HF
- BclI-HF *
- BsaI-HF V2 *
- BspHI *
- BsrGI-HF
- EagI-HF *
- EcoRI-HF *
- HindIII-HF
- KasI *
- MfeI/MfeI-HF
- MluI-HF *
- NcoI-HF
- NgoMIV *
- NheI-HF 4SE *
- NotI-HF *
- PspOMI *
- SalI-HF *
- SpeI/SpeI-HF
- XbaI *
- XhoI *
- XmaI *
Blunt cutters:
- AfeI *
- BsrBI*
- DraI
- EcoRV *
- FspI *
- HpaI *
- MscI *
- NaeI *
- NruI-HF *
- PmeI *
- PmlI *
- PvuII-HF
- ScaI-HF
- SfoI *
- SnaBI *
- SspI-HF
- StuI *