Divide and Conquer Biological Challenges
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Contents
- 1 * We need to get the Lux/AHL system working.
- 2 * We need to get the Lsr/AI-2 system working.
- 3 * We do NOT need to use additional chemical input signals (e.g., aTc and IPTG). We will use only AHL and AI-2 added exogenously.
- 4 * We need to have an XOR logic gate produced.
- 5 * We need to figure out how to get cells to communicate in a sequence and not stop growing too soon.
* We need to get the Lux/AHL system working.
* We need to get the Lsr/AI-2 system working.
Our summary of the Lsr system: Davidson/Missouri_Western_iGEM2008#Lsr_.28AI-2.29_cell_signaling_system
- There are NO Lsr parts in the registry:
- We will need to make BB parts for:
- LsrR and LsrK which are adjacent to each other in the E. coli genome. We could amplify them together with a total size of over 2600bp.
- Lsr promoter
- LuxS that produces DPD that somehow is converted to R-THMF.
- How do we get cells to make AI-2?
* We do NOT need to use additional chemical input signals (e.g., aTc and IPTG). We will use only AHL and AI-2 added exogenously.
* We need to have an XOR logic gate produced.
Existing Logic Gates
- NOT:I732205 (NOT/ Dual-repressed NOR)
- AND: (insert links to examples here)
- NAND:I732914
- NOR:I732916
- Inverters:J23040 (AHL-dependent inverter)
Q04121 (lac inverter)
Q04400 (tet inverter)
* We need to figure out how to get cells to communicate in a sequence and not stop growing too soon.
- What if AmpR is secreted and cells are not AmpR? This would prevent cells down the chain from responding too soon.
