Davidson Missouri W/Davidson Protocols

1. How to Keep a Lab Notebook
2. Common molecular reagents
3. Standard Assembly
4. BioBrick Ends
5. Compatibility of Plasmids
6. Building dsDNA with Oligos
7. Setting up PCR mixtures
8. PCR and Mg²⁺ concentration
9. Clean and Concentrate DNA (after PCR, before digestion)
10. Pouring an agarose gel
11. Calculate MWs
12. Digest DNA with restriction enzymes
13. Double Digest Guide
14. Ethanol Precipitate DNA (short protocol)
15. 1kb MW markers
16. Shrimp Alkaline Phosphatase
17. Qiagen QIAquick Gel Purification
18. Qiagen QIAquick Column Regeneration Protocol
19. ElectroElute Gel Purification
20. Ligation Protocol
21. Heat Shock Transformation OR Short version of Heat Shock
22. Zippy Transformation
23. Colony PCR to Screen for Successful Ligations
24. Promega miniprep
25. Choices for Transformation: Heat Shock vs. Zyppy
26. Choices for Mini-Preps: Promega vs. Zyppy
27. Sequencing at CUGI
28. Making dsDNA Using Primer Dimers
29. Gene Splitting Web Site
30. PCR Primers w/ BioBricks
31. List of auto-inducers and their Sigma catalog numbers