LasR/cI
From GcatWiki
Goal:
pBAD+RBS+lasR+TT(James DC)
S0388?(I13453+B0034+C0079+B0015)
pLasR/cI+RBS+LuxI+RBS+Mnt+TT
K091141+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015
Ligation Plan
| Vector | Insert | Product | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Step | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Size |
| 1 | K091141 | RBS+Mnt+TT | R/X;S/P | R/X | 468+2079 | C0261 | RBS+luxI | R/X;S/P | R/X | 661 | TBD | |
| 2 | K09114 | plasR/cI | R/X;S/P | S/P | 164+2079 | TBD | RBS+luxI+RBS+Mnt+TT | R/X; S/P | X/P | TBD |
Parts
| BBa_ | Description | Source | location('07) | Vector | Responsible |
|---|---|---|---|---|---|
| B0015 | double terminator | registry 07 | Plt.1-1I;Plt.3-3O | pSB1AK3 | |
| I13025 | RBS+Mnt | registry 07 | Plt.1-23J | pSB1A2 | |
| K091141 | plasR/cI | MW | N/A | pSB1A2 | |
| C0261 | RBS+luxI | registry 07 | Plt.1-24A | pSB1A2 |
