Suppressor Logic Toolkit and Quasi-Hash Function
Contents
Suppressor Logic Toolkit and Quasi-Hash Function
The following slides describe the basic toolkit design and a specific experiment that would result in a quasi-hash function
Basic Toolkit Design
The AND gate was designed by Anderson using termination suppressors, while the new design would use frame shift suppressors,
![Picture13.gif](/GcatWiki/images/3/3a/Picture13.gif)
![Picture14.gif](/GcatWiki/images/c/c9/Picture14.gif)
![Picture15.gif](/GcatWiki/images/5/5f/Picture15.gif)
![Picture16.gif](/GcatWiki/images/3/3d/Picture16.gif)
Toolkit with Specific Inputs and Outputs
PprpB/propionate and luxO/pluxT are new communication players that would need to be looked into further to verify their usefulness.
See references at bottom of page.
![Picture17.gif](/GcatWiki/images/5/5f/Picture17.gif)
![Picture27.gif](/GcatWiki/images/d/dc/Picture27.gif)
![Picture25.gif](/GcatWiki/images/8/8d/Picture25.gif)
![Picture28.gif](/GcatWiki/images/0/00/Picture28.gif)
Experimental Design
![Picture21.gif](/GcatWiki/images/a/a9/Picture21.gif)
![Picture22.gif](/GcatWiki/images/0/07/Picture22.gif)
Parts Needed
Promoters
pR R0051
pLacIQ1 K091112
pTetR R0040
pLuxR R0062
Genes
RBS+lamba cI+TT K082022
pBAD+RBS+LacI+TT S03980
pBAD+RBS+tetR+TT K091145
pBAD+RBS+luxR+TT K091179
RBS(B0030)+GFP E5500
RBS(B0030)+RFP S04120
References
Powerpoint File:Suppressorlogic3.ppt
Anderson et al. 2007. “Environmental signal integration by a modular AND gate” [1]
luxO luxT [2]
PprpB propionate [3]