Suppressor Logic Toolkit and Quasi-Hash Function
Contents
Suppressor Logic Toolkit and Quasi-Hash Function
The following slides describe the basic toolkit design and a specific experiment that would result in a quasi-hash function
Basic Toolkit Design
The AND gate was designed by Anderson using termination suppressors, while the new design would use frame shift suppressors.
![Picture13.gif](/GcatWiki/images/3/3a/Picture13.gif)
![Picture14.gif](/GcatWiki/images/c/c9/Picture14.gif)
![Picture15.gif](/GcatWiki/images/5/5f/Picture15.gif)
![Picture16.gif](/GcatWiki/images/3/3d/Picture16.gif)
Toolkit with Specific Inputs and Outputs
PprpB/propionate and luxO/pluxT are new communication players that would need to be looked into further to verify their usefulness.
See references at bottom of page.
![Picture17.gif](/GcatWiki/images/5/5f/Picture17.gif)
![Picture29.gif](/GcatWiki/images/9/90/Picture29.gif)
![Picture30.gif](/GcatWiki/images/f/f3/Picture30.gif)
![Picture31.gif](/GcatWiki/images/8/85/Picture31.gif)
Experimental Design
![Picture21.gif](/GcatWiki/images/a/a9/Picture21.gif)
![Picture22.gif](/GcatWiki/images/0/07/Picture22.gif)
Parts Needed
Parts from Registry
Promoters
pR R00512009 Kit Plate 1 6K pSB1A2
pLacIQ1 K091112MWSU
pTetR R00402009 Kit Plate 1 6I pSB1A2
pLuxR R00622009 Kit Plate 1 6O pSB1A2
pMnt R00732009 Kit Plate 1 16B pSB2K3
Genes
RBS+lamba cI+TT P04512009 Kit Plate 2 1N pSB1AK3
pBAD+RBS+LacI+TT S03980MWSU
pBAD+RBS+tetR+TT K091145MWSU
pBAD+RBS+luxR+TT K091179MWSU
RBS(B0030)+GFP E55002007 Parts Kit Plate 1 9J pSB1A2
RBS(B0034)+RFP MWSU
Mnt C00722009 Kit Plate 1 12L pSB2K3
LuxO-P K1310222009 Kit Plate 3 8K pSB1AC3
Parts needed to be built from scratch
prpBp Ecocyc
(text version)
tgaaattaaa catttaattt tattaaggca attgtggcac accccttgct ttgtctttat caacgcaaat aacaagttga taaca
http://gcat.davidson.edu/GcatWiki/images/2/2f/PrpBp.JPG
prpB 2-methylisocitrate lyase in E.coli MG1655 [1]http://gcat.davidson.edu/GcatWiki/images/6/6c/PrpB.JPG
pLuxT [2]
http://gcat.davidson.edu/GcatWiki/images/e/e3/LuxT_upstream.JPG
frame shift suppressor 1 CUGUCCUAA
frame shift suppressor 2 UUACUAGAC
prpBp and prpB have no EcoR1, Xba1, Spe1, or Pst1 sites. Source www.neb.com
References
Powerpoint File:Suppressorlogic3.ppt
Anderson et al. 2007. “Environmental signal integration by a modular AND gate” [3]
luxO luxT [4]
PprpB propionate [5]