Blasting Spacers for Our Genome

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Revision as of 16:37, 10 November 2009 by Karicheson (talk | contribs) (Blasting Spacers)
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Blasting Spacers

CRISPR one results from CRISPR finder are shown below. I blasted all of the spacers using the nr/nt database and the results are shown below.

CrisperOne.jpg


Spacer One: TGCGTCGTCCGGTGGCCGTCAATAAATGTCGCAAGGG

SpacerOne.jpg

Matches by chance!

Spacer Two: TCCTACGACCTCGTCGGCGTCAACGGCTGGCCCGA

SpacerTwo.jpg

Spacer Three: CACCCTACAACAGGTGAAATCTACCAGACAAAAGA

SpacerThree.jpg

Spacer Four: TCACCCAAGCGCAAGCAACAGCTGATCGAGGACCTG

SpacerFour.jpg SpacerFourTwo.jpg

Spacer Five: GCGACGGCGGCCAGTTCCGCGAGGGCGGGAAGGTCC

SpacerFive.jpg

This image is only part of it. . .

SpacerFiveTwo.jpg


NOTE: lots of hits from each organism- indicates that multiple parts have been incorporated into our species' spacers. . . interesting to study what genes have been inserted

Spacer Six: TGCGAGTGTTGCGGGGAACCGACTCGGGTAGGCCAG

SpacerSix.jpg

No viral insertions in this spacer

Spacer Seven: ACGGTTTCGCTACCACCATCGCCACCAGCAACTGCCG

SpacerSeven.jpg

Spacer Eight: GACGAGTATGCCAACCGGCTCGTGAGCGGGCGC

SpacerEight.jpg

E-values not good- no significant hits!

Spacer Nine: TGGTAGGCGTCGTAGGTGTTCGTGGCGAGCGTGTC

SpacerNine.jpg

Spacer Ten: ACTCACTGGATTATGACCCCTACAACGAGGGCGTCA

No significant hits. . . lowest E-value is 1.5

Spacer Eleven: GGATCTCGATCGTTGTAGTATCCATAGCTGCTATACC

SpacerEleven.jpg

Spacer Twelve: GAAGTAACGCAACTCCAGTGAGCGCTACTGAGAGCCC

No significant hits. . . lowest E-value is 1.6

Spacer Thirteen:CCGATCACGCCCTGCCGATACTGGTAGTTCGCGATA

Spacer13.jpg

Spacer Fourteen: TCGTCGGCCGGCTCGTCGGCCGACGTGGACTTGC

Spacer14.jpg

Spacer Fifteen: AGTAGGTCTAATGTCTCTCTGTCGTCTATCAGCCCCG

No significant hits. . . lowest E-value is 1.6

Spacer Sixteen: GCTCTCCGGTGTCACAGGTCAGGTCACGGTCTCCGC

No significant hits . . . lowest E-value is 1.5

Spacer Seventeen: ACGGACAAGTCATCCACCCGCCAGTATCTCCCGGT

No significant hits . . . lowest E-value is 1.4

Spacer Eighteen: AAATACGATCCTGCGGTGACGCTACGTCCGGGGCAGC

No significant hits . . . lowest E-value is 6.4

Spacer Nineteen: CAGATGTGGGGTCTGTGGCCACAGTCTAACATCTCT

Spacer19.jpg

Spacer Twenty: CCTGATAACGGACTCTTGTAGGTCCGTTAGGTCGT

No significant hits . . . lowest E-value is 22

Spacer Twenty-One: AAAAATGAGTGACGTAGACATTCGGCAAAATGCCGG

No significant hits . . . lowest E-value is 6

Spacer Twenty-Two: CAGCAGCGAAACGAGCCGTCCGTCCTTTTGAGACA

No significant hits . . . lowest E-value is 1.2

Spacer Twenty-Three: GTCTAGCCCAGTCTGGTCGGGGTGGTCGGCAGGATCGG

No significant hits . . . lowest E-value is 0.43

Spacer Twenty-Four: CACTCCTCATATGTCTGTTCGAGCAGCGGGACGTG

No significant hits . . . lowest E-value is 1.4

Spacer Twenty-Five: ACCGTTGCCGCCGATCGGCAGCGAGCCGGTGATGTGT

Spacer25.jpg

Spacer Twenty-Six: TCAAAGCGAGCCTCGAACGCGACGACGAAGATATG

Spacer26.jpg

Spacer Twenty 27: TCCTCCTTGTACCCACGGTCTTGCCGATCCATCCCG

No significant hits . . . lowest E-value is 6.0

Spacer Twenty-Eight: GACTGGCGTGTTGCCGTTCAGGCCGGCGTTGATCCCG

File:Spacer27.jpg

Spacer Twenty-Nine: CTCAGCAGCAGTCAACGGCATTTTATACACCTTGT

No significant hits . . . lowest E-value is 0.36

Spacer Thirty: CACCCCTTCCGGGGAGACGAGGAAACCCCGGACGA

No significant hits . . . lowest E-value is 1.4

Spacer Thirty-One: GTCACGCTGTCTGACGATATGGCTGACCAGGTGC

No significant hits . . . lowest E-value is 1.3

Spacer Thirty-Two: CACTCCTGGGCGGCCTCATCGGCGGCCATCGTC

Spacer32.jpg

Spacer Thirty-Three:GTTGTGTGAGGTATGCGATGGACACCACCGATCACG

No significant hits . . . lowest E-value is 6