File:ThyA on Amp plates.jpg
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Revision as of 18:13, 3 June 2014 by Tetaye (talk | contribs) (We took the transformed cells with the thyA fitness module and plated them on just Ampicillin plates. This was to mitigate having to select for both kanamycin and ampicillin resistance. Only transformed cells were expected to grow and they did in compa...)
ThyA_on_Amp_plates.jpg (311 × 543 pixels, file size: 18 KB, MIME type: image/jpeg)
We took the transformed cells with the thyA fitness module and plated them on just Ampicillin plates. This was to mitigate having to select for both kanamycin and ampicillin resistance. Only transformed cells were expected to grow and they did in comparison with the negative control plated on amp. The negative control is just the plasmid that carries the kanamycin resistance, so it would have needed kanamycin to grow.
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current | 18:13, 3 June 2014 | 311 × 543 (18 KB) | Tetaye (talk | contribs) | We took the transformed cells with the thyA fitness module and plated them on just Ampicillin plates. This was to mitigate having to select for both kanamycin and ampicillin resistance. Only transformed cells were expected to grow and they did in compa... |
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