Ethanol Precipitation of Vector DNA
From GcatWiki
- After digestion with restriction enzymes (ex. Normally a 40 µL digestion)
- Add 1/10 the digestion volume of 3M NaOAc pH 5.2 (ex. 4 µL of NaOAc)
- Add 2X the reaction volume of ethanol (ex. 88 µL of ethanol)
- Vortex and put in -80°C freezer for 15 minutes
- Centrifuge for 15 minutes (Place the tube hinge out in the microcentrifuge to know where the pellet is.
- Dump off the liquid and allow to dry, either sitting on the bench or with the lyophilizer.