Nanodrop DNA for Bio113
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Revision as of 15:29, 3 January 2020 by Macampbell (talk | contribs)
Nanodrop DNA
- The Nanodrop will be blanked using the elution buffer from your mini prep.
- Pipet 2 µL of your mini prep onto the small open stop on the metal circle.
- Carefully lower the arm and click on the measure button.
- Record these values for each of your samples:
- absorbance value of your DNA at 260 nm
- concentration of your DNA in ng/µL
- 260/280 ratio value