Test Mnt/Tet promoter
From GcatWiki
Test Mnt/Tet promoter
The pMnt/TetR promoter is constitutively on and is repressed by the TetR protein. The addition of aTc should repress the TetR causing it to glow. The addition of the Mnt protein should repress the promoter causing it not glow.
Test tet repression
Some promoter (pbad)→RBS+TetR+t+t+pMnt/Tet→RBS+GFP does not glows
I13453+RBS+C0040+TT+K091105+E0240
add aTc
Some promoter→TetR+t+t+pMnt/Tet→GFP glows
I13453+RBS+C0040+TT+K091105+E0240
Test Mnt repression:
Some promoter→Mnt+t+t+pMnt/Tet→GFP glows
I13453+RBS+C0072+TT+K091105+E0240
Test Tet and Mnt repression:
Some promoter→TetR+Mnt+t+t+pMnt/Tet→GFP does not glows
I13453+RBS+C0040+C0072+TT+K091105+E0240
add aTc does not glow