Sequencing at MWG Operon

How to Activate SimpleSeq Samples and Order DNA Sequencing

1. Go to www.operon.com and log in.
2. In the “Order Now” drop-down menu on the right-hand side, click “Order – DNA sequencing”.
3. If you already have your SimpleSeq tubes, click “Activate SimpleSeq Reactions”.
4. Enter either the barcode located on the side of the tube rack or a barcode from any tube in the rack. Click “Activate”.
5. Close out of that window.
6. Select your appropriate “Format” and “Sample Format”

• • • In this lab, the “Format” is usually “Tubes” and the “Sample Format” is usually “Premix DNA Template and Primer”***

1. For each DNA sample you’re submitting, enter:

• “Barcode”: the barcode number of the tube.
• “Sample Name”: the name of your sample.
• “Template Type”: select “Plasmid” if using miniprepped DNA
• “Size (bp)”: the size of the whole vector including the insert

1. When you’re finished, click “Submit Samples”.

• • • NOTE: The system will automatically detect that the barcode belongs to a SimpleSeq tube and will enter a no charge order into the system ***

1. Review your order and click “Proceed to Shopping Cart”.
2. If you are satisfied with your shopping cart, click “Checkout”.
3. Select your shipping address and billing address and click “Next”.
4. Enter your payment information and click “Next”.

• • • In this lab, we pay with our PO number. Retrieve the PO number from Dr. Campbell.***

How to Submit SimpleSeq Samples

If your sample format is “Premix DNA Template and Primer”: a. Mix 8 μL of template and 4 μL of primer for a total volume of 12 μL b. Make template and primer solutions that match the concentration ranges listed below: