TBLASTn and Protein Sequence Analysis

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When Nucleotides Don't Work

Occasionally BLASTing genes with just a nucleotide sequence doesn't work. This can be due to a number of reasons:

  • Lack of conservation between species
  • Incomplete genome database
  • Rearrangement of introns/exons

If, for any of these reasons, you aren't getting any hits, it might be time to make sure you're doing it right:

First -

Beyond blastn

Why Amino Acids?