TBLASTn and Protein Sequence Analysis
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Revision as of 18:38, 22 February 2011 by Dycoughtreydavenport (talk | contribs) (→When Nucleotides Don't Work)
When Nucleotides Don't Work
Occasionally BLASTing genes with just a nucleotide sequence doesn't work. This can be due to a number of reasons:
- Lack of conservation between species
- Incomplete genome database
- Rearrangement of introns/exons
If, for any of these reasons, you aren't getting any hits, it might be time to make sure you're doing it right:
- First - Check to make sure that your database is made correctly.
- You should have three additional files made from the original fasta file:
- First - Check to make sure that your database is made correctly.
