Pouring an Agarose Gel

Pouring an Agarose Gel

1. Go to http://www.bio.davidson.edu/courses/Molbio/Protocols/molwt.html to determine the percentage of agarose and calculate amount of agarose for 35 ml.

1. Weigh out agarose in 125 ml flask (eg. 0.35 grams agarose for 1% gel).
2. Add 35 mL of TAE buffer
3. Microwave for 1 minute
4. Swirl and microwave for 10 seconds
5. Let flask cool enough to touch, add 5 µL of 10 mg/ml Ethidium Bromide (WEAR GLOVES)
6. Swirl to mix and pour into gel tray with comb