Malcolm C
This is where I will capture my progress on this project.
I will also be working on the 13 genes Allan gave us.
SSR Primer Hunt
The class has already completed 4 SSR primer designs (LDOX, CHS, StSy, and F3H).
Results from Blueberry 454 Scaffold Searches from Grape (given to us by Allan Brown)
AOMT Scaffold 00796
- Forward Primer GAGCTCTTGGATGCTCCATACT & Rev Primer CCTCATAATTTTTGGTCTTCGC (taa) x6 PCR product = 137 bp
- Forward Primer TATCCCATGTTTTGGCTAATCC & Rev Primer TTTGCTTTTCAATCGGGAAC (tc) x9 PCR product = 261 bp
AOMT scaffold 01781
- Forward Primer GGCGACTGTGTCTTTCAGTAAA & Rev Primer TAGGATTTCGAGGAGGAGAGGT (ct) x7 PCR product = 136 bp
- Forward Primer ATAAACAGGTAAACCGAACCGA & Rev Primer CCGATTAAATTGGAGCAGTAGC (tg) x7 PCR product = 262 bp
ANPER scaffold 00666
- Forward Primer TTTCTATCTCTCTCGCCGTCAT & Rev Primer GTTTTCTCACCTTGTTTTTGCC (ct) x21 PCR product = 219 bp
- Forward Primer CCGTTTCGATCTCTCTCTCAGT & Rev Primer CATCCCTAGATCTGACACCACA (tc) x14 PCR product = 278 bp
CHI scaffold 03186
- Forward Primer TACATCTTGAGGGGCAGTTTTT & Rev Primer GTGGAGTGTGGGATATGGATTT (tc) x5 PCR product = 121 bp
- Forward Primer ACGTGGTCCAATCTAACCTCAC & Rev Primer TAGTAAGTGGAGGCCGGTAAAA (cttt) x4 PCR product = 288 bp
DFR scaffold 03132
- Forward Primer TACATCTTGAGGGGCAGTTTTT & Rev Primer GTGGAGTGTGGGATATGGATTT (tc) x5 PCR product = 121 bp
- Forward Primer ACGTGGTCCAATCTAACCTCAC & Rev Primer TAGTAAGTGGAGGCCGGTAAAA (cttt) x4 PCR product = 288 bp
F3'H scaffold 01438
- Forward Primer CAGTGGTACAGTGGTGGATGAC & Rev Primer ATTCCCTTTCTCTTCCCATTTC (ag) x7 PCR product = 152 bp
- Forward Primer ATACCAATCCATAGTCTCCCGA & Rev Primer TCAGTTGTGTTTTTCAGTTGGG (ta) x7 PCR product = 279 bp
FLS scaffold 00142
- Forward Primer CAATTATACCCTTCCTGTCCCA & Rev Primer GAATGGATGAAAGGAACCCATA (ag) x8 PCR product = 214 bp
- Forward Primer CTAGCTCTCCCTCTCTCCCTCT & Rev Primer GTGGTACTCCATATCCATGCCT (ct) x8 PCR product = 295 bp
FLS scaffold 00374
- Forward Primer TGAGCATCATTGCCAGAGTAGT & Rev Primer GGATTTCATTCCAAGAGATTGC (ct) x6 PCR product = 295 bp
- Forward Primer TAGATTATCCGGGGAATCATTG & Rev Primer GATTCTCATGACAAGCCAGTTG (tc) x6 PCR product = 275 bp
MYBA1 scaffold 03500
- Forward Primer GCGAGGATGGAAATATATACGG & Rev Primer GCTTCATAAGCTGCTAGGCAAT (ag) x8 PCR product = 222 bp
- Forward Primer GCGAGGATGGAAATATATACGG & Rev Primer GCTTCATAAGCTGCTAGGCAAT (ttat) x5 PCR product = 222 bp
MYCA1 scaffold 00278
- Forward Primer CAGTATTTGAGCCGGAACCTAC & Rev Primer CGTACAGAGTTTCGCACAAAAG (ct) x7 PCR product = 268 bp
- Forward Primer TAGTGGGAACGAGTCGTCTTTT & Rev Primer AGTTGGTGCCCTTTTATGAAGA (at) x6 PCR product = 195 bp
MYCA1 scaffold 00554
- Forward Primer GGCTAAAACCCTAGTTGCCTTT & Rev Primer GGGTGTACTGAGAACCGAAGTC (tc) x6 PCR product = 157 bp
- Forward Primer CCCAAAAGAAAATACCTGGACA & Rev Primer GGAGCTTGGACCTAAAAGGATT (ga) x8 PCR product = 260 bp
UFGT scaffold 00304
- Forward Primer TGAACTACCAAAATGGGACAGA & Rev Primer NGACGGAGCACATCCTACTACA (ag) x10 PCR product = 207 bp
- Forward Primer CCCCAAATTCAGATCAAAGAAG & Rev Primer CTTATCTCTGCCCACCTACACC (ag) x12 PCR product = 230 bp
UFGT scaffold 01120
- Forward Primer TGTGTGTGTGTAGTGTGGTCGT & Rev Primer AAGAGAGAGGGGTCCTGAAAAC (ct) x11 PCR product = 132 bp
- Forward Primer TAAGCTACAGTACCTCGCCACA & Rev Primer AAGGATATGCCGACGAGTCTTA (tc) x6 PCR product = 196 bp
EST Search Results
For the second half of the semester, we focused on finding ESTs of our genes using the Washington State University Vaccinium genome database as well as the Towson State University Blue Berry Genome database using 454 sequencing.
ANPER - anthocyanin permease
AOMT - anthocyanin O-methyltransferase
CHI - Chalcone isomerase
DFR - dihydroflavonol 4-reductase
F3’H flavonoid 3' hydroxylase
LDOX - leucoanthocyanidin dioxygenase
Myba1 - myb-related transcription factor
MycA1 - myc anthocyanin regulatory protein
UFGT - UDP-glucose:flavonoid 3-O-glucosyltransferase
1) ANPER
Submitted the 3’ most blueberry sequence from my scaffold00666
ggatggcaagctctggtagcttacatcaacttgggatcctattacat
Got this result from BLASTn search of Vaccinium database
>DR067294
Length = 260 Score = 60.0 bits (30), Expect = 9e-11 Identities = 39/42 (92%) Strand = Plus / Plus
2) AOMT 01781 search of Vaccinium database
Submitted carboxyl most amino acids that gave hits with my scaffold01781
Did tBLASTn
EEMVLEDLRPSRESTMKLNKMLAADPRVMISHASIGDGITLCMRV
No hits.
I submitted
ATCGAGCTAATATTTTATGAGACcACTTAAAAAaTTTAAAAAaTTATTTtAATATTAATGAACGATTCGGCTCATTTGTACTCTACGGA
From the scaffold
And got
>DW043195
Length = 523 Score = 48.1 bits (24), Expect = 7e-07 Identities = 33/36 (91%) Strand = Plus / Minus
3) AMOT 00796 search
submitted
ATCTAAAAATAAATAAATAATTTGAGTAATAATTATTAAAGACGAAAAAaTAAaGATGACGAAATAGTAAAAGCTTTGGAAGATAAATTTTCAAACCTGGAAACCCGCAAATTACTCCCACTTGCATATATAAGTACTCATATAATTTTCTATTATTTAATTTAATGGGTCCTAACATGATTGTAGTTATTATTTTTTTTtATTATTATTGTAGTTATTTATACTACGCAAGATGAAGGGTGCACAAGTCTTCCATGTTGTGGAATATCGCAGCTACAAGCATTTATTTAATGGGTACAAAATATTTTTGTAAGCGTCGtGCCCATTATAGGTGAGCATATAAGTCTATAAAGTATTAATTTAGAGAGGTCAATTAAAAAaGAaTCTCCCTGTGACACTACAATCGTGCTTGATGTCAACAAaTATGaCAAAAAAaTATATaTAAAaTaTAAAacAAgTACTTtCAtgcATtATTtaTTtAAtATTTttCAcACAAATTTGAAATTGATGCCGAAAAATTAGAAATTTCACATAAAATATTTATTTAAaTTAAAAATAGCACGGTTTTTCATAAGGAACTAAAATTACTTGGCGGACGGAATACCAGAAAATCACGCAATTAATTCTTCAaGTTTTTCACTTTTCACGGAGGTGGGTTTATGATCtCGTAAATAAGTAGTTTCTTCTTTTTTtATTGTGGATATAATCTCCTTCTATTTTCTCTAAATATTGAGGATCATGATAAtGCAAAGATGCAAGTGGTGGCTAGCTCATGCGGTAAGAGCTCTTGGATGCTCCATACTGCATCCCGAGATCGAGTCCCATCTCCCTTCCTAATGTAATAAATCAGGAATGATGAATGAAAAaTAATACGGAGTAATAATAATAATAATAATGCGAAGACCAAAAATTATGAGGATTTTTTtAGGAGGGTTtCGAATTACACCATCcTTTTTGGTAGGTGAATGGTTCAGTGCATGTGAGCATTTTAATAAAATCATTACATATTTTGGtATCAAATGGTATTAGTATATATAATTAATTAATTAAGTAATTATGACCCCTTGCATGTGAATGAAGGTGGTGGTGGGACCACCAACACCAATACCAACTCAACGGCATATACATATTGGAATCGTCAATAGcGGGCAAGTGTGGTTGGTGGAaGgTTGGTGAAATGAAACTTTaGtCcACGTAACAaGAAAaGAGTTGGTGGCCATCCCcTTCAAGGGATATCATTTTCTTTTtAAAGGGCATaTAAAAAAAAAaTtAAAaTcACTAACAAGAAATTTTAAGGAAAaTAAaCAaGCCCAACcaTAAAACGGTAgATACCAAaTCTATACCACCTTATAGTATATCTATTTTGgAATTtCACACTAgTGATCAAACGGaTGTGGGgTTgCTGCTATGCAGCCCTAGCACAACAAGTGCTGTGTATTTGGCTTGTTCGTTACTGGGATGGACggCCcAGATTTTACCtCGGCACCTTTGCATCGAatCGTTTGTtGTCGTGCGTGCAAGTGAAATCTGGATCGTCCATCAGAGCAACGGATGACCCGGATGTGCATAGCACCTGCTGTGCACGGGGgTgCACAACAGCAACCCcAGTTCTGATCAAACcAATTCTATTATTTtAAaGATTATTTTATGGgTCTTtGAAaGAAAAAAAaaCTCATTCGGATGTCGTTAAGGGCTTAATAGGACGAAAATCGATCAAGCATTTAAtGATATtTGAATGAGTTGATTTTTTataGagACTCTtAAGAAtaTGTTTTAAATCAATTGAAtGACTCTgaTCATTTTTGTGGAATTCCAAAATAATTTTtACACGATAGGGTAAATATTTGGCaTACACCACATGGTATAaGCAtAGTTTTTTTTTTTttGgtGCCTTTACTTGgTTCATTTCCTTTATTTTTGCGAGTCTAAGTCATTATCGTTTCTAGGGATGGCAACGGGTAAgaCATGGATCAGGGATCCTATTTTACCTTCGATCCTTAAATGGGAATTTCCTTTCCACCCCcACcTCCATATCTAAAAGGGaTAGTgTTttCCAcgATACCCGCCTCCCAATGGGGATCGgaTACACTtGGGTAAATtcATATCCAAACTAGTTGAAAAAaaGAACAaTAAGATCTAAAtAAAAATAaTTtaCgTGTCC
BLASTn
Got 3 EST hits
4) CHI
I did BLASTn with (from scaffold near SSR primer) TTTCTTGAGGTCTTCCAGGATGAGACATTCCCCCCTGGTGCTTCTATTCTTTTCACCCAGTCACCCCATGGGTTGCTAACGGTCAGTACATCCTTCCACAAGTGTTAGTTCTGCCTTTCTCTTCGCCTTTtGTTCTCTCTTCCTTGTTGATTATAAAATAGGTAATGCAGTTCCTTGATTCAGGAACAATTTAAGCAGGTATCCAAATTTTCTTTtGAAaTTTGAGGgCAAGGAAATCACGATGATTTATGAGACTGATGTTGCATGTTAATTtCAGCTTGTACCCCAGATGCAGCATCGATCAGGACATTGTGAATTTCTTTtGTTTTtGGTCACCATTACTTGGTTCTCTACTAGTTgACGGTTCACTGTGCACTAaGCTCCATTTTGATGGACTTCATAAAACaCGATGCAGGACAagATGTAAACCAGGCCCATGAAAATAGTTtCGTGTTTTAAATCaTTAAGCCTGTTCTTGACTTCGCCAAGAAGAtCAAACTCGTAGTATAAAGTATGTTGTTGAGAAGtGTTCTGAAAAGTAAGcAAATGGGTTTCTATTTtGGTTTTCATCTTTTTATAACACTCTTGGTTAATTTTtACCCTAAAAAaTACTCcATTAaTtAAGACCTAAAGACAAcTGGTTTGGGTtGATTATAGGTTTACTTTTATCTGTTtGGTGACATCACTAGATaGGCTGTGTTGCGACCTTGCCGATTGGTTTtCACAATCGGAGCACCTGAGTTATGAAGAGACATGCTTTTTtGAGGAACTTTAGGCCCGATATCGTCTTAACTCTTGAGAATCATTTTCTTCATTGATTTGGACATACACACACATGCATTCGAATCCGTGGTGCAAATGATTCTGTAAGATACAGGTCATTTGAATCTTCATGCTCAAAACCCCCATTCAATTAGCGACTTACTTCTTACTTCCCAAGGAGCAGGAACTGCTCTTTTAACCCTTCTCCGAGGATTACATCCACAAACACAAAGTCGATGGCAGACTGATATTACTCATCATCATTATCTATTGCATTTATTTTTCTTGTTGCTGATCATATGTATTTGACAAACTTTGGGATTACATGAGTATGTACATCCACAGATATGCATTGGTGTTGCGCGTGTATGTAGGAAGTTGCAATGAAACAATAACGTTTTGATTTCCTATTGTTGATGCTTTATTTTCTGACTACAGATTGGCTTCTCGAAAGATGGTTTGTTACCTGAGACTGGCAAAGTGGTAATCGAGAACAAACAATTGTCCGAAGCAGTGTTGGAATCAATCATCGGGAAGCATGGCGTTTCCCcTGCAGCAAAGCAGAGTTTGGCGGCAAGAGTgtCTGCATTGTTCAAGGAGGCATAAGAAAGAAGGTTTTGGAAAACGATAAGAAAGAAGTCCTGTAGCAGAAAagttAgTAGtAGTTtATTGAgagaGGAAGATAAAATGGTAGAATCTCACTTCCCAGTTGATTCTGATGTTTCTCCATGAGTAAATGTATCTGTACGCTCTTTCATTTCCGTTTtGTCACTGTAATAAGAAGGCTTCAaCTTTTGCATTCTAAACTCATCACATCTATCTACGACCTTTTCTTATTCTTCTTTgCAAATTTAAACCAAACcGagCCTTATGtCCCAATAaaTTGGGgTCTACTATTGGGgTCTACTATGtAAatCCcTTTTTttGtCcATTGGgATCTGtGCAAATTCAAATGATATTGAAATTAAaTTCGGCATAaTGGCGGTAGTGGTTATGGACATCATGAATTAGTAATGTCAAGTGTGGAAATTTTtGCTTGTTTtACTTTGtGTAATGCAAGATGTCGAGGCCAGTATACAAGGGTGGAGCCAAGATTTTAGGTAAgCGGGGtCAAAAATTAAACGCATATGCAATATAtATTTTTTCtATTTGCTTATGCAAAGAAATAACTAATTTGAGAGTTTAACTATAACGTAAATATACAATGATGCATGTATTTCTGCTTTATAATTGGTCCCAATGACTTATTATATCTTGAAAAaAaTCACTTATTCAGATTTTTtACTTATTTAATAAAAAAaGTGAGATTATATtAGCTTCAATTTGTAGTTAATTTATTTTAAAGTACTTGAAAATGTTAAAAAaGGTTTGACCAAAAaGTTTATTTATTTTCAAGAGTGCTTCTATAAATTCCTTAAGACAGGTAATTAATTAGAAGTTATTCTACAACTTATTTAACCAAATAATTTACACTCTTCTTTTAACTTATGAAAGAGCACCAAGCGTATCTATAAATTATTTATAATTTTTAACTTTATACTTCTAAAAaTCATCATTTAAAATTTtAACTTATGAAGGAGCAAACAGCTGGgATTGATTTAAAATCTTCGGGACTAGTAGAGCCGCAAGGCCCGCAAAGACTTGCTTGgTTTTGCATACAGATTGCCCATTTGATTTCCTTTTTATGGGCCTTGTAATTTGGTCTGATTTAATCAATAGAAACTGCGTTTGTTCGTCCTAAAAAAaGGTTagATAAAGATCCTCTTATTTGATGATTTCTATTGTGATGCAAAACGAAATTACATAGCAAGCTCATGTATGTATTGGAGAGTTCAACACTTTATAGCTTTTTACGATAACTCGTATGTCTGGGCTAGTTTCAGTTCACCTCGAGtAATTTTGGGGACcAGTCGACTGTTTACTAGTGAGAGCTCCAATTAAAGCTAAAGAAGCTCCGTATGAATTGACTCAAAaGAGTTGAAAGCACCTAAGATGTTTCGATCCTAAAATCTTAAAAGGAAGCAAACCCCTAAGTTTTAGGGTCATCAGGCCAACTGCTTAGGgTTAACTATTTATAGCTTTTAACTTGGGGAGGTTGTAATGGTTATTTACAATGATTAAAaGAAAAaTTATGCATAACGACAAAtCCAATAATTAAAAGCTTCATAATCCCTTCTCTCTCTAAAACGAAATCCTCTCACTCTAGAAaCCAAAAACCCTTATTTCTGTCTTtCCTTTtCCcTTtCCCCcTAGGgCTTCACCCATGCGGCAACAGTGTAAGAACCcGTGTTTTTAAATGATTTTTCAGAAATAATGTTGGGAAAATGTAAGGAAACgAAAATAGATTAAAGTGAGCCAATGTGGTGTTAATAGATTGCATGTGAGTGCATTTAGAACACATTAGAGACATGTGGCGTGTGTGTGCAATTAAGGGTGTGCATATACATCTTGAGGGGCAGTTTTTTTTTtAAAaCtAAAaCACTTCTCTCTCTCTATAGGgCCcGCTGCCCCcTTTCTCTCTCCcTCTCCATTTCTCTTGCCAAATCCATATCCCACACTCCACCTCTTTAAAATCTAACCTTAAATCCTTCATCTATGCGTGATTTGGAGTCCGAGTGTCTTTGGTTTCACTTGGAGgAGGTATATTTtCGGTTGAAATTCAAGAACAAAAgTTGGGGTTTCATTTCTCACCAATTGGAAGCTAAATCTTTGAGGTAGTGGACAAATTTCTACTCCCTcTAGTTGTATTTTGTTGATTTGTCGCATATACCATTGTGTTGCCCACAAATTtCTAGTCAATCGTGAAATCTCCCTAGAAATGGGTTTGGTTTTGATTGAGTTTGATTCCCACTTGTTTGGTTGTTAAATTCACAAATTGGTGGTTGATATACCCTTATTCCATGCTTAAATGATATTTGAAATTCCAAAATTGACATGGGTTGGAACCCCCCTTCATTTGTGAATtGCTGTTAAAAATCTCATTTTGTAGcAGTAGATACCAAGTTAGTGAGGTACGATACCTTTTGGTTTTGTTCACTGATGGCGCGTTGGTATCTATACCAAGTTGGTGAGATATGGATACCTGTTGGTTCTGTTCATTGACGgTGCACTGGCATCCATACCAATTTGGTGAGGTATAGATACCTTTCTTTCTGGTCGCTAAtGGTGCATTGGTATCGATACCACTCTTGGAGGTATCCAtaCCTTTTTAAAAAaGTTCCAAAAAACTCTCATTTGGTTTCCGGTTGACTTATTTGGTATCATACTTGATATATTCACCCCGTGCACTTTATTTTTGGTCGAATTGCGCCTTTCTAACCTACTTtCATATGATTGGTGATAaTGTTGATCTTCCAACGTACTTTGACACATatAAACTTTGTAGAAATTGGCGATCTACGTTACATGAAATGAAAATGTATGATGTTTGTTATTTGGACATGTGTGAAATACTTGGTGATTTttGAACGAAAAaCAAGTTGATGCACTAGGATCACTACTCAGTGAGTCACTAGGAAAGGTTTCACGAGGCCCATAAGACTCGGACAAGGGTTCTACGAGGCTCATAAGATTCGGATATGAGTTCCACgAGGCGCATAAGACTCGGACATGGGTTAAGACTCGGACAAGGGTTCCACGAGGCCCATAAGACTCGGATAGAGGTTCTACGAGGCCCaTAAAACTCGGACATGGGTTCCACGAgCCCATAAGACTCGGACATGGATTCCACtAGGCCCAAAAGACTCGGAAGAGAATTTCAATATTCTCAAGCTTGAATTGATGTACACAtGAGATTGATAAAATGTGAAAATGGTTGAaCGTGGACATTTATGGGATACGCGGATACTCAGAGACACAGTCGGGTAGTTTACGGgCATGTTGGGTTTTCTGTTATCATATGTGGGTCCTATATGGACATTTGACCATATTTTTACCTTTGGACATTTGTTGGTCATTCGTCACATGTTTCGAATATTtCGTAGTATCCATTGGTTGTATCACACATGATAGTCATTCTCTTATGCACATATCACACTTTATACACCTAACGTTTGTAGAATGGTTTtCCTAATGGGCTAGTGAGCTCACCCTATCTTTTTCAGGTACATGGCAAGAAACATGTGTTGGATGCTTGGAGTGTGGATGCATtGCATGAAGCTTGTTTGATCATGAAGTCATTTTATCCTCCCCATACTTAGTATATTTTTGGGAGATGATCATGTAACTTGTGAACACCCCGCcGGAGTTCACGAAAATGGTTTAAGAAAGCAGTTTCGATTgATTGGA
Got
>CF810723 Length = 647 Score = 60.0 bits (30), Expect = 1e-08 Identities = 85/102 (83%), Gaps = 1/102 (0%) Strand = Plus / Minus Query: 4838 gctagtgagctcaccctatctttttcaggtacatggcaagaaacatgtgttg-gatgctt 4896
||||||||||||||||||||||| |||||||| ||| | | |||| | | |||||||
Sbjct: 112 gctagtgagctcaccctatctttcccaggtacacagcatggagcatgagatcagatgctt 53
Query: 4897 ggagtgtggatgcattgcatgaagcttgtttgatcatgaagt 4938
|||||||| |||||| ||||||| || | ||||||||||||
Sbjct: 52 ggagtgtgaatgcatgccatgaagattttatgatcatgaagt 11
>CV190427 Length = 537 Score = 58.0 bits (29), Expect = 5e-08 Identities = 35/37 (94%) Strand = Plus / Plus Query: 1616 aaaccaaaccgagccttatgtcccaataaattggggt 1652
||||||||| ||||||||||||||||| |||||||||
Sbjct: 45 aaaccaaacagagccttatgtcccaatcaattggggt 81
5) DFR
I submitted the cDNA from grape ATTTTCTTCCCCGGATCATTTTTGAGAAGTATGGGTTCACAAAGTGAAACCGTGT GCGTCACCGGTGCCTCCGGTTTCATCGGTTCATGGCTGGTCATGAGGCTCCTGGAGCGCGGCTATACTGT TCGGGCCACCGTTCGCGATCCAACTAACGTGAAAAAAGTGAAGCATTTGCTGGACTTGCCCAAAGCGGAG ACGCATCTGACTCTCTGGAAGGCAGATCTTGCTGATGAAGGAAGTTTCGATGAAGCTATTAAAGGCTGCA CCGGCGTCTTCCATGTTGCCACACCCATGGATTTTGAATCGAAGGATCCTGAGAATGAAGTGATAAAGCC AACTATTGAAGGGATGTTGGGCATAATGAAATCGTGTGCTGCCGCAAAGACTGTCAGAAGGCTTGTATTC ACATCCTCCGCAGGAACTGTGAACATTCAAGAACACCAACTGCCAGTGTACGATGAAAGCTGCTGGAGTG ATATGGAATTTTGCCGGGCTAAAAAGATGACTGCATGGATGTACTTTGTCTCCAAGACACTGGCTGAGCA AGCTGCATGGAAGTATGCCAAGGAAAATAACATTGACTTCATCACTATCATACCGACTCTTGTGGTTGGC CCCTTCATAATGTCATCAATGCCTCCAAGCCTCATAACTGCTCTTTCCCCGATCACTGGAAACGAAGCTC ATTATTCAATTATACGGCAGGGCCAATTTGTTCACCTGGATGACCTCTGCAATGCTCATATTTACTTGTT TGAGAATCCTAAGGCAGAGGGACGTTACATTTGCTCCTCCCACGATTGTATCATTCTCGATCTTGCAAAA ATGCTTAGAGAAAAATACCCCGAGTATAATATCCCCACAGAGTTCAAAGGTGTTGATGAGAACTTGAAGA GTGTCTGTTTCTCCTCCAAGAAGCTGACAGATTTGGGGTTTGAGTTTAAATACAGCTTAGAGGACATGTT TACTGGAGCTGTGGACACATGCCGGGCCAAGGGATTGCTTCCCCCTTCACATGAGAAACCTGTAGATGGC AAGACCTAGAAGAAGAATTTCTGTCAGGCTTTCTAGCGAGAGCGTAGAAATGTCCTTCATTCTGTGGAAT CAAGGTTTTCTGGTAGTTTGATTTGGCCAACTATGTATGAAGTTTGTCTACTATTTTATCCATTAAAGGA AATTATTCTAATGGTCAATGTGCCGGAAATCAGAGTTCGTTC
>DW043076 Length = 475
Score = 79.8 bits (40), Expect = 3e-15 Identities = 187/236 (79%) Strand = Plus / Minus
Query: 562 gccaaggaaaataacattgacttcatcactatcataccgactcttgtggttggccccttc 621
||||| ||||| |||||||| ||||||| ||| ||||| || | ||||| |||||||||
Sbjct: 407 gccaaagaaaacaacattgatttcatcagtattataccaacattagtggtaggccccttc 348
Query: 622 ataatgtcatcaatgcctccaagcctcataactgctctttccccgatcactggaaacgaa 681
|| ||| | || | || |||||||| || ||||| || ||||| ||||| ||||| |||
Sbjct: 347 attatgcctacattcccaccaagcctgatcactgcactctcccctatcacaggaaatgaa 288
Query: 682 gctcattattcaattatacggcagggccaatttgttcacctggatgacctctgcaatgct 741
||||||| ||||| || ||| ||||||||||| ||||| || || || || | ||
Sbjct: 287 cctcattactcaataatcaagcaaggccaatttgtgcacctagacgatctttgtgaatct 228
Query: 742 catatttacttgtttgagaatcctaaggcagagggacgttacatttgctcctccca 797
||||| ||||| |||||| ||||| | |||||||| | ||||||||||| |||||
Sbjct: 227 catatatacttatttgagcatcctgaagcagaggggagatacatttgctcatccca 172
>CV091566 Length = 829
Score = 46.1 bits (23), Expect = 5e-05 Identities = 86/107 (80%) Strand = Plus / Minus
Query: 715 gttcacctggatgacctctgcaatgctcatatttacttgtttgagaatcctaaggcagag 774
|||||| ||||||| | |||||||||||||| ||| | ||||||||||| || || ||
Sbjct: 509 gttcacgtggatgatgtatgcaatgctcatatatacctttttgagaatccgaaagctgaa 450
Query: 775 ggacgttacatttgctcctcccacgattgtatcattctcgatcttgc 821
||| | || |||||| | || ||| ||| || |||||| ||||||||
Sbjct: 449 ggaagatatatttgcgcgtctcaccattttaccattctggatcttgc 403
6) F3’ H
submitted from scaffold (near SSR primer)
ATCAGCCAAAGTCACCAGTCACAGACTATCAGCATGCACCCTGGGCCAAGATCAAACTGTCCGTGTGCAATTAAGGTTTGATTATAAAATCCCAACAGTTAAAAACTAAGTAGTCATTACATCACTTtGGCTACTTATATATTCACCTAGATAGAATCAACCAACAGGTTcGTATGGCGATTCAGAGACACATGAAATACCATTGATGACAGGATATAAACACACCAACACAAATGCCTATtAATACAAATGAAGAGCAAGCATGAATTTTGTGCACATCATAAGTTCATTAGCTAGGgCATAAATGGATACAATCTAAACGAGTCCTCAAAGTGCCGGCATTTtGTCTTGTTTTTGTCATTATTGTAAGAGCTGCTTCCTGCTgATAGTGCATTcTCTCCAAACTAAACCTACTAGGTACAAAACCTTCATGGCTTCTTCAAACCTGGGTGGCTACCAAAGTACTACTCACCATTACACTAACTGTACTGACAACTTTCTTTATCTTGTTAAACCTGCTGAATACTTATCATTTGTAGCTTGTTGCTTATGTTAAAGTTGAGCTTGTTAACAAGCGGCAAGGCTTGTACCATGATTGGGTTACTATGAGACTAAGAGCACATACACGTCTACCGGCCTCCATGTGACACAAAGAGCAGAGTGACAATGCAAGGAGGTAGTGCATAAATGAAGCTTACATTGGAACTTTGTAAAGTTTTAAACTTCAAAAGATGTGTTTTCTAAGAGTTATGTCTAACTTTTAGTTTTGAATGAAAAAACATGACTTTGCGAAAAGATGTTTAGAATCCTATAAATATAGGATCACCCCACTTACTTAGCCTAAATGACTTTTAATTCTAACAACTCTCCGTATTATTTTCATATATTATGGGcAaTTcGATTCCTTCTTGGCACCCCCGTCTtCCACTGATCGTGTAACCTGGAGACAAACTGCGAGATCTATGGAAGGTATCTTGGAGTCGTATTCAGCCTTTGGAACCTTCATGAAGGGAGGCAAATATGGCTTTAGGATTTATCCAAAAATATGGCTTTAGGACAGcGATTTGTCGTGCCTTTCATCGATTtCACATGCTAGTTTCCTAATTCTATTCATATTACATATTTGTGTGAttGTGTTCATATTTTTAGTGTTCAATGGCATTTCTAATTTCCTAGTGTTTTGTGATAATCATGTGACAAAaTCCCAACAGCTTATTCCATTCTAAGGCAGTAGTGAAGGAAACGACAAGTCAAAAAaTATGGACACAACCCGACACTCTACGGACAaCTGTCAAATACACCTAAATATGTGTCTAATTTTTCTGTACTAAATTTTCAAATGAACATGTTGTGGACTCTATAGGAAAGGTtGGGGATTCTCTGGGGCACGTTCAAGACACATAAGGAGTTAAAATGCAACTTTTAAAAGTGGAGTCAAAACTTCCATTGCTGTTGAGTACATTAATATTAATGTAGTAACCATTAATTGGcATAATGTTAATACTATTGGTGTTTTTTGTGTTAATTtGATAGAAATAATCTTTTAACTTTTATACGAATTCCTACGAATCATTGACTGATATAAATATACATTATATATtCCACGTGCCcTAACGTGCCCgAaTCTTAGTTTTTACGGAGAAGACATCCGTGTCCATTTTGTGTCCATATCCTTGCTTCTTAACTGTTAGATAATGACGATcAAAAATTCGATCACTCCAAcGATTGAGACACTGCGTATAACACCAAACTCTCCACCAACTCTCTCACAAGTTGATGTGTCCCACACcTGCGGGGCTTGTGCGAGGGAAATTGAGCTGTTACAGTCTAACTAACGAATGCCAATTCAGTGGGCTAATATTTAAGTTATTGTGTTCGATTTCTATGTTGTACTTCCATAAaGAAAAATTGGTGCATCTGTTGGAGTTTGTCCCACATTGGTTAATTAAAACCTCCAAATCTAGTATATGAaTCTGGGCAGCCTCTCCACTCATTGCCAATTGGTTTTGAGTTGGATGCATTAACATGGTATCAGAGCTAAGCTTTCgCAGGCTTTGGACCAATCTCTTGATTGATTGCCcTCTTgTtaGTGCGATAAAGTGCACCTTGTTTCAATATAATCCAATGTTACGGATCTTTGTTTACCTCTCCCCATGCGGGAaCTAGACAGGGGTCGCACATGCGGAGAGAGCATCTAGATCTCAAGGCAAGTTGAAGTGAGaaaaaaaaaaagaaCAGTTTGTGATAAGCAGCAGAACTGAGACATCAGATGCTTGCTTAAAAAACACCTTTCGATGTAATAAACAGATGGGCTGCCAAGCATGTGAccTTTTAAAACAGCaGTTAATAACATAGAAATCGAAGGTCTCCTATCTCACTACAATATCTATCCCCTAGACAATCCcCCTAAGTgTCTCTTtCGAGAcaGTTTTCACATTATTATTGTTATTGTtCCACATTGCTTGGGTATGGAATGGGAGATCACTTAATAACATATGGAACCTCTCCACTAATTGCCAATTGgTTTTGAGTTGGATATAAAGTTTCCAAATGGTATTAGAGTGGgCCAATTCCATCCCAAATTTCTTAACAAAAaTTAAATCAAGCCCACACCCCACGATGACAGACCCAGGAAAGGTTGTACgATGATAGAACCCAAAAATTGGCCACAGGTGACAGACCTAAATGTGGCTGCAGGTGAGGGGGGAGTAAGAATAGTTGTCTCATATTGCTTGGGTATGGAATGGGAGATCACTTAATAACATTTGgAaTCTCTCCACTCATAGCCAATTAGTTTTTGAGTTGGATATCCACAATTATTTCAACATTGACTCAACTGAGCACCTTTATCAATCTTTCATATCGACTCCCGTGTACAAGAGGCAAATCAGTGCAAAATCACCATAATACATAAAGCCAAAGCTTTCAAATATTCAACCACACCTGATATAGTCTCAAAGCATTTGAATTTCCAATTAGAGCTTGCAGATTAGACTTGGGAAGATCCAGCAGTTGTTGCACATTTGAGAAACCCCTTCTGCTTAGGGAACCGAAGAGATCATCAGTCATGCATGGCAACATCCATAGAGCAGTGTCACTGCCAAACCATAAACCCTGCAAATGAATCCAAAAATCCATCAAAGTGTTGCATGAAGAGGAGAAAAATAGCAGAACCCACAAAACTAAACCTTCAAATGGAGGCTGTTTAAATCTTCTTGATGGACAGAATAAAAAGAAAGACAAGTGTAAGAACCAGGACACAGCTCCAGGTTATTTCCAGGAAACTATTACTGCAATGGATAACATGGAACaatttATGTTCGAGAAAGAAAACCAGGTCACCTGTCCTGCAAATAAcTTTAGAAGTAGCCAATTTCCAAGGGACCCCGTGaGAAACCCTCGGCCGTCCGATGGTGTTTTGGACGGCCCGGATGTGTTAAaCACATTTGGGCCaTCCAAAACACGATtGGACGACCGTCGATAAGAGTGGTACATAAAGAATCGTTTATTCCCACAAGAAGATGCCTATTCAAATGGTCGAATACAGTTCCCTAAAAGAACTTACAACTTACAAACTTTGATAATCATCCCTTAGCTGTGGAAAATAGGTCCGATTCACTTTCTTACCGAAGGGCGGGgAGGATAGATTGATTCAtCTAGCCTGTCAAGTAGAGCCCCCTATAGACGTCAAGACTTGACGGCCTAAATGGGTGTATCATGCCTTGGTtATTGATCCCAAGGCCCAATCCAGGATGAAAACTCCATCTATAGATCTCTAATGAGgACACAACCTTAACAcGATGGCCAAAAaTAGAAGCTTGATGCGTGAAACTTCTGAAGAAAAAATTAACCCAAAAAGTTGATGTGTTTCGAGAAACATAAAGAAGCAATGGCTAAAATGACAAGATGATATACTGGTAGGTACAATAAACTACAGtGGGTAAATACAACCAACGAAGAGAGCCTCCAAGTTTAGATCTCCCTATTGAATATTGGAGACCAATTCGTTGCTGCCAAGGTCAAAATTTGAAAGAGGCATAAACTAACCTAAGAATAGAACAAAACTTTTTATTTTGACTTCTGGAGCTTTGGAGGTCTAAGATAATCCCAAAGTCCAGCGTGGTTTCTCTGGTCACAGAgaGAAATTTTTCCTAAACgaTACAAGAGATAAAGATGAcAACTATTAACTACCAACAGGGATAAAACCATGGTAAAAATACAAACTATACATAGATTTTTGGGTTATGGTTTACCCACcATTTTAATAAGTGCAAGTTCAGGAAAAGGTATACATGTATGTACCTACCCATTAATTCAAATTGCTGCACTGATGCGATTAACACAGAAACAGAATGTACTAGAAATCTTTCACAAGGGAAAAAAaTTTGCTTGGCCAGAATCGACAAAGGCAAGTCTAGTAGTACCAGCAATAACTTGGTCAATACAGAAAAATGCAACTTTTGCATCTTTTCTGAAATAATTGTACTATATTCCGCTTGTTTAGGTCTGTTTCGCTATTGGgTTGTCTTTTTGTGTTCAATGGAAACATAAAGCTTCATAATtCCACCCGTGGTTCCTTTCGGTCTCTTAGGAAATGGAAAGAGTTCAGCCATTTCTACAGAAACAAATATGTATTAATTTCATACTTCAACGAATGTCGCTTTAACgACATACCACAAATTCACCGATGACTTAATTATTCTGGATTCACAATGACTGACTTAGCATCATATCGAGAAGTGCCTAGCAGTATCTGTGAAAGACCAAACATGTATTCcTGTCCTCATAGATTgttGGTACAAAACTTGAATTGATCGGGAAAGAAGTGCCTGGTtGGAAATCATAGCAAACAAACTATAGTAACAGCCTCAAGTGGTGAAGGCATTCCCAATGTAGATCCTGAAGAaCTTATATCAGACAAATGTTGCATTAACAGTCAAACTACAAAGTTGAACATTTTGTACTTAGTTGCATGATGGACAATATTTTACCTATCATTCGCAATGAAACTGGCAAAATCACTACATGGGCGAAATAAATAATCAACTCTTCTAACTAGTTCAATgTAATTTCAGACATCCAGCGTCCACTGTCCACTCCCAATTTTCTATAGaTATGAATAGGATTATTTCATACGACATGATAAAAGAGGTCATCATAAAAACGACGAAAAACTCCTCATAAGAACATTCGGcAGAATGCAATATATTGTTTAAAAGCAAGCTTTTAACAACAGCAACCATGTGATTGATGAGACTGATGATTAAGATGGAAAAGACAGTACATACAGAATTAGAATCACCATTCTATTCTTATCTATCAGACCGTTCATCTACTGGGAAAAAGCAAAAAAGCAACGACCGGGAGAAATACCTGCATGACCATTTGAAGAAGATGCATACAGGTGATTGTACTTGAAAGCCATCCACTGTTCGCACATATATCTATCATTGCCTGTATGATACGTATACTTTGATCGAGCACAGATTTCAAGTCTGTGACATAGTCTGTGATtGGTAATTCTAACTGAGAAAAGTGGGCCTGCAGACCAAACATCATATATTTAAGAAGATAAGACCCCAAGAACAAACACAGAACCATGTATTATATCCAGAAGACAGTCAAAAAGTGATGTCGACCTGGAAAAGTAGATTCGCTTTAACATGTGGATCATCTAAACGATTCTTATCCACCATATACGGAACTTTCACAGACAATGCTTCATTGTAGTTTTCCTAGAACCAAAGAGAGGATGATTAGAGAAAAAGTCAACAGACATTTCATGTGTTATTAATATAAACTTTGAAGATGAAGCCACGACAAACAGCCAATAAATCATATAGCCAATAAAAGAGTGCATCATCTATGTGTCCAAGAATtGGATTCATCGACTTCTCAAGCCCAAAGACTAGGGAACATCAAACATTCAGATTTTTTTAATATCATTCTTTCATGTGCTCTGGAAAAACTCTCTTCACAAAaCATAGATCATTACGCATAGTTGTTCAGCtcTTTtTTTTTTTtAAAaGCAACAATGTCAAGgAaGTAGCACTGTTCCTGAGTTAAAATATACACCAACACGTTAAACTGAGACAGCGAGAAACCCTtCCTGGTTGGTGAAAGCAAAATCAGAATTTATTAGTTTGATAATTAGCAAAAAAAAAaGATGATCACAATTAGAAAACCACCTCATTATGCCTCACAGGAAGCTCATCATATTCGGAAGAACCAGACAGTATGTGAAGGAAAACCTGCAACGAAAAACAAATGAAGTGAAACATTGCTTAAAAAGGGATGAAAATGGAAGTTATACCGGCTATATGTTGGAGTTTTTACTTCAAGAGATGTATCCGGGCCTATATTTGAACCAAACATTGAAACAGTCATGTACTTCAGGTAATACTGAGAAGCTATTGAGCCCAACATCATAGGTTCCACGCTGTCTTCATTCATCTTCAAACACCCACTGTCTTCCAGATCCTCAAAGGTGTTTTGCACCAAGCTGCCAAACAAAACGGTTATAGAAAATTGCACTACAACAAAACCTTGGGTCATACAGTAGGTTATTACCTTGACAAATATGAACTCACAATTTCAGGTCCAGTATCCtCTAAGCCATAGTAAGCTGGATTAACCATCTGTCACAGGGATATATTTTTTGTAAGAAACTTTAGCAGGGAAACAAAGACAGCTGAAAGCCCAaTGCTGGGTATAGAAGCTGTGGTGTGATTCCATCCTCAGTCGATCATTGTTCCAAAGATGAAAATATCATAAACATGCAcAATTGAACAcTATAAATGCATCTAGTCCATTATTTCAGGGAACATGACatGCATGTTTTGGGCAATCACTACAATCATTACGCTGAAAATGCAAATTGAGAAtAGCAGGTATCCAGGTCAACTTCGTGCTTATTATGAGGCATACATGGCATCATTATTTTGCTAATAATGTTAaGCAAGAGAGTGTCTCTACGCATTGCTTAAGTTGAGACACCAAATCGAGCCTTCCACATCACcGAACTCGATAATCATTATAGACTAACAGAGTTTTAGTAATTCACTCCATACGCTCTGCTTGTTATCATCTACATAATTATATTAATGGGTTTGATGCCTTAAATAAATCTTTCCAAACAGCCGGGAAATAACCAATATTTTACTAGATTTTAATCTCAACTTCCAAGGGTACAAAACATTTAAAAAATATTTGGTATTGTACATGACCAAATAAGTACAAAACAGTTTCAAAAACTTTTCTGTTGTTTTCTCAAAATTGGGTTGTCTTTAGAGAGAAATATTGTTTGCCAAACAAGATTGGTGTTTAATGGAAACGGAAAATAGAAAAGCAAAACAAaCaaaacaGGTGATTAGCTACCATAAGTTACAACTACTGTTCAACTTCAAAGAATTTAGCATTTCTGTTTAAATTAAAAGAATCTAATGTTAATCATGTGAAGAAACCAAAGAAGCaCTTCCAACAGCTAAACTAACACGGAAAGTTAACAACCTGGTCATATGCATTTTACATTTTCCCCATTTTACAATGTGCTCCCACAAAAATGTGAAAACGTGTTCTTATGTAACCTCTCTTCAAGTTTCGGATACAGGACTCTGCTTGTTGACATCAACCTTGCATAGATGTTCAAAATATTTTCTGCCATTATAAGACAAAAGAAGACCATTTTTTCTCTTCCCAAACTTTTGTATGCATCTTCCCAATATTTAAAATGTGCCTCACATCCCAAGTGTAAGTGTGTGTGTGTTTAACACTGGATGTtCTTTCCTGATAGGTGTAAGTGAATGTAATCAACATAAATATAGTTTGACAaCCGAGAGAATGTCCAATATATCTATAGAACACAAAACAAGAACTCCAGTCTCtAGTACACGCTTTATCCTTCTGAAATGAGTATAATAAGAAAATGAGGGgAAGCCTAGAATAAGTCAGGACCAAGCAAGGTGCTTTAAGCgCTGATTTACTTATTGCGCATAAAAAGGAATTGCcGAAATTTTACCAATCTCCGAAACAAATAAGTCCAGGTGAGATAATGAATTGCATCCTCTCTATGGCAAATTGTTCCAGAAACTATCTCAGCATTAATGTGGTCATGCAACTGCTCCCTCAGACTACTTTCAACTGGGAATGGTTCATACAGAAACTGCAACAAATAAAAC
>CV190608 Length = 474 but this is for RPC-1 which is an RNA polymerase 3 so I don’t think this is right
Score = 139 bits (70), Expect = 3e-32
Identities = 111/125 (88%)
Strand = Plus / Plus
Query: 1924 gagtttgtcccacattggttaattaaaacctccaaatctagtatatgaatctgggcagcc 1983
|||||||| |||||||||||||||| ||||| |||| ||||||||||| ||||||| | |
Sbjct: 137 gagtttgttccacattggttaattataaccttcaaagctagtatatgagtctgggcggnc 196
Query: 1984 tctccactcattgccaattggttttgagttggatgcattaacatggtatcagagctaagc 2043
|||||||||||| |||||||||||||||||| ||| ||| |||||||| ||||||| ||
Sbjct: 197 tctccactcattaccaattggttttgagttgaatgttttaccatggtattagagctaggc 256
Query: 2044 tttcg 2048
|||||
Sbjct: 257 tttcg 261
7) FLS submitted from scaffold near overlap sequence with query GAGAGAGAGGgaGgTTGcTGCAATCACGCTCTAGTATCGGTtGTGAACTCTGCCGCAGGAGAGGTGCGAATGGTAGGTGACGTCagCGGCGATGCGaTGCTGGACGCcGGTGATGGGACTGGGAGGGGCTTGGGGTTTTGAAGGACCAAAGGGAGTAGACCATTAGGAGAGTtAGGTGGAGGTCCAAGAGTGTCGTCGTTTGGGTCTGGTGGGTTGTTTCGCCATGGAGAGGTGGTGGTGGTTGGCAGTGAGAATGGGAGAGCTCAGGCGACATTATTTTTGAAGGATGTTTtAGAGGTGGTGGTTATtAATATgTGGgTTATGTAGAGGAGTGGGgACGGATACGACACAACACTCTCCGGACACGTGTTGCTTAAATACTTGTCtAATTTTTtCTTGTATTTCTTTTATTCAAACACATGGTAGATGTTCAAGGACACGTTGGCAAAACTCATAGAGCGTGCTAAGGACACATAAAAGGTTAAAATaTAATTATTAAAACTtGGCCATAGGTGAAAATTtCCACCGATGTATAGTCATGTATACTTAtgCgCAGACTGCTAATTTATCTTTGTTGtaTTaTTTCTAAATTAGATAGAAATAAAGATTTCTAATTTATATGAATTAATCACATACCTCTATGATACAAaTAAaTTTtATATTGGACGTATCCCCAACGTGTCCTTGTCCTAATTTTtGAAGAAATAACGTGTGTGCGTATCCGCATCGTGtcGTGTTCGTATCCCgTATCCGTGtCcGTGCTTCTTAGACAATAAATATTGATCgTCGGTAGCCACACAGTTTACTAAGGGGCAAAATGTCTAACTAGTTGGATTCAaCTATAGCAGTAGCCGCTAAAACTCTGTAGGCTCgCTCTCTACTCCTATGGTAGCCTATGCTGCTAACTCAGTTGAtGGaTAGCATGGATCCcATAGAGCTCTCTCAGTCTCTAGCCTTGTTTATGGCTCAAATCCCTCCTCATCTCCAGTTTCACATCACCGGACAGCTATATAGCCAAGCCTATTGTATTTTTtGTATGAACACTATGGAATCTTTCATTCCgAaTTCAACACGGCTATCCAGACGGAACCTGAAGTGAGGCTTTAATCTGGGTTCTCCCATACGTACCCTTTTtATTGAGAATGCAAGGCATTTACGGATTGACATCCAAAATAAGATaCAGACATGGGACAAAGCTATGTGAATGCCGTGTtAATTAATTGGGGAGCTATTCCATTAGTATTAATTTTGTATACCACAGAAACTTCTTGCCTCTAGTtGTCCACTTTTCCCTctcATCCTCTCATTCTGGGTGACCCTTATATGTGCATATATATAGATATCAACCCACAAACGCAAGGTTGATTAGTGATCATAGAAaGAAAAAaTGATGGACCAGAAGGAGAGAGTGCAGGCCATTGCCTCGGTGTCAACAGACACCATACCGGCAGAGTTCATCAGGCCAGAAAGTGAGCAGCCCGGACTCACAaCCTaTGGTGGGCAGGCCcTTCAAGTCCcGGTCATCGATTTCTCAGACCCGCACGAGGAAATCCTCGTGGGTTCCATTGTCGATGCAAGTTGCcAaTGGGGAATGTTCCAAATTGTGAACCATGAGATTCCAGTAGAAGTAATAAGAAAATTGCAGCAAGTTGGgAAAGAGTTTTTTGAGCTCCCACAAGAAGAGAAGGAGGCTTATGCTAAGCCTGTTGGGTCAAAGAGTGTGGAAGGGTATGGAACAAAGCTGCAAAAGGAAGTGGAAGGGAAGAAAGGTTGGGTTGATCATTTGTTCCATAAGATATGGCCTCcTCATACTATTAACTACCAATTTtGGCCTAAAAaCCcACcTTCTTACAGGTTCgTGATaCATTTtCTTCTCTCTCGATTATTCgTTAGGGgCAGATAGAAGGgCAAACAaTTAGAGTACTACTGTTTTGgCAAAAAaaTGGATtATTTTTTTGTCTTTATTCAAaTAAAATTTGCATTtgTtAGTTTTGCGTTGATTTTTTGTGGATTATTagTTCATCTCGACAAGATGAATCTAAAAAGTCAAGAGTTACTATCAAAattaaaaaaaaaaaGTTCaCTAAAGACAATAATtCAAAATTTtCTCAAAaCACACATTCATGCGATTCGACATTtGTCATTACGCATGCGTATaTTGGGAaCATcTGATCTGCaTTTtttATTttGgTtATTTTtCTTGGTGTcTTATAAAAATGTTGtGTCTtGTt
>CV091044 Length = 735
Score = 363 bits (183), Expect = e-100 Identities = 306/342 (89%), Gaps = 5/342 (1%) Strand = Plus / Minus
Query: 371 tgcttaaatacttgtctaattt-tttcttgtatttcttttattcaaacacatggtagatg 429
|||||||||||||||||||||| |||||||||||||||||||||||||| ||||||||||
Sbjct: 571 tgcttaaatacttgtctaatttgtttcttgtatttcttttattcaaacagatggtagatg 512
Query: 430 ttcaaggacacgttggcaaaactcatagagcgtgctaaggacacataaaaggttaaaata 489
|| |||||||||||||||||||| |||| ||||| |||||||||||||| ||||||||
Sbjct: 511 ctctaggacacgttggcaaaactcttagaacgtgccaaggacacataaaaagttaaaatc 452
Query: 490 taattattaaaacttggccataggtgaaaatttccaccgatgtatag-tcatgtatactt 548
||||| |||||||||||||||||||||||||||||||| |||| ||| ||||||||| |
Sbjct: 451 taatttttaaaacttggccataggtgaaaatttccacctatgtgtagttcatgtataagt 392
Query: 549 atgcgcagactgctaatttatctttgttgtattatttctaaattagatagaaataaaga- 607
|| ||| ||| |||||||||||| |||||||| || ||||||||||||||||||| |
Sbjct: 391 at--gcaaactcctaatttatcttcgttgtattgttcttaaattagatagaaataaatat 334
Query: 608 tttctaatttatatgaattaatcacatacctctatgatacaaataaattttatattggac 667
||| | ||||||||||||| ||||||||| ||||| ||||||| |||||||||||| |||
Sbjct: 333 tttgtcatttatatgaattcatcacatacatctataatacaaacaaattttatattcgac 274
Query: 668 gtatccccaacgtgtccttgtcctaatttttgaagaaataac 709
||| |||| |||||||| |||| |||||||||||||||||||
Sbjct: 273 gtaaccccgacgtgtccgtgtcttaatttttgaagaaataac 232
8) LDOX submitted from scaffold AAGCTTCGTTTAATTTTTCAATATATAAGAAATGCCAAAAAATGGTACATTGAGACAGTGGAGAGGGAGAGAAATCCAACCAAAAGTTAAATAAGAAAAAATTAAAAAACCACCCAAACATTAGACACCCGAGTACCCAACGGCCTCAACCTTATCGCCTTTATCGGTGAGGTAGAGACCGTGTCAATGCCACCGCTGCAACCCACCACCAACAATCGTTTTAAGTTGCATCCAATTATGTTTATTTCCATAAACTAGCATAAACATTTTTCAATTTTTAGTCTACCTTTTAGGTATGGAATTTTTCATGATGTTATCTTGAAGTTTGGCTCTACCGACTGACTAAATTTTCGTGGCTCCGCCCGCGCGCAGGTGCCAACCATTGATCTAGAAGACTTGGTATCGGAGGACAAAGAGGCCAGAGAGAGATGCCACGAGGCGTTGAAGAAGGCGGCGACAGAGTGGGGGGTGATGCACCTAGTGAACCACGGCGTCCCCGAAGAGCTGATGGACCGGGTTAGGGTGGCCGGAGAGGGGTTCTTCAATCAGCCCGTGGAGGAGAAGGAGAAGTATGCTAATGACCATGATACTGGGAATTCTGGGAAGATACAGGGGTATGGGAGTAAACTGGCAAACAATGCCAGTGGGCAGCTGGAGTGGGAGGACTATTTCTTCCACACTGTTTACCCTGAGGACAAGAGGGACATGAAGATTTGGCCCAAGAACCCATCCGACTACATGTAAGTTTTTTCTGAATAACGTCCATAGATTTGCTGTTTACATTAACGGGTCCGAACATGATGTATTTAGAACCATTCTACTCCGATAAACCTGGTGGTATAGAAAGGGGCGTACAAAAAATGTACACAGCGCGTTTGGGGACCCATTTTTGGGTTCCACATGAGTGATCCGAACCGTTCATTGTTCTAAAACATTTTTCTGAGGGGGATCAAACAAATCAGCTCAATCGGACGGTTAGAAGTGCTTGAACCAAAGTCCCACCGTTTCAAATAAAAACTTTTCATTTGAAACGGTGGGACTTTGGTTCAAGTACTTATTATAACCATCCGATTGAGCTGATTTTTTATGGGCCCCCTCGGAAAAATGTTTTAAGAACAATGAACGGTTCGGATCACTCGTGTAGGACCCAAAAGTGGGTCCCCAAATGCGCTGTTTACATTTTCTGTACACCTCTTTCTGTACCGCCAGCAGCGTCCGTTCTATTCAAGTAGGAACTACAAAAATTTATTCCACTAAGACCGCACATATCTCGAGTAATTTTGAACTTAATTTGCAGTACAATTTACTAATGTTTTATGCACCG
>CV190548 Length = 671
Score = 65.9 bits (33), Expect = 5e-11 Identities = 40/41 (97%), Gaps = 1/41 (2%) Strand = Plus / Plus
Query: 701 gggacatgaagatttggcccaag-aacccatccgactacat 740
||||||||||||||||||||||| |||||||||||||||||
Sbjct: 3 gggacatgaagatttggcccaagaaacccatccgactacat 43
>CV191078 Length = 651
Score = 65.9 bits (33), Expect = 5e-11 Identities = 43/45 (95%), Gaps = 1/45 (2%) Strand = Plus / Plus
Query: 697 aagagggacatgaagatttggccca-agaacccatccgactacat 740
|||| |||||||||||||||||||| |||||||||||||||||||
Sbjct: 2 aagaaggacatgaagatttggcccacagaacccatccgactacat 46
9) MybA1 did tBLASTn using amino acid results from scaffold03500 LGVRKGAWTEEEDCLLKKCIEKHGEGKWHQVPYRAG
>DR067494 Length = 369
Score = 43.5 bits (101), Expect = 2e-06 Identities = 18/35 (51%), Positives = 24/35 (68%) Frame = +3
Query: 2 GVRKGAWTEEEDCLLKKCIEKHGEGKWHQVPYRAG 36
G+ +GAWT ED +L + I +GEGKW +P RAG
Sbjct: 144 GLNRGAWTAMEDKILTEYIRVNGEGKWRNLPKRAG 248
>CV091396 Length = 791
Score = 41.6 bits (96), Expect = 8e-06 Identities = 16/34 (47%), Positives = 22/34 (64%) Frame = +1
Query: 3 VRKGAWTEEEDCLLKKCIEKHGEGKWHQVPYRAG 36
++KG W EED +LK +E HGEG W V ++G
Sbjct: 19 LKKGLWKPEEDRILKNYVETHGEGNWASVSQKSG 120
>CV191530 Length = 673
Score = 39.3 bits (90), Expect = 4e-05 Identities = 16/32 (50%), Positives = 21/32 (65%) Frame = +1
Query: 5 KGAWTEEEDCLLKKCIEKHGEGKWHQVPYRAG 36
KGAW+++ED L I +HGEG W +P AG
Sbjct: 148 KGAWSKQEDQKLIDYIRRHGEGCWRTIPQAAG 243
>CV090776 Length = 717
Score = 38.1 bits (87), Expect = 9e-05 Identities = 17/34 (50%), Positives = 23/34 (67%), Gaps = 1/34 (2%) Frame = +1
Query: 3 VRKGAWTEEEDCLLKKCIEKHGE-GKWHQVPYRA 35
V+KG W+ EED LK+ I+KHG G W +P +A
Sbjct: 103 VKKGPWSPEEDAKLKEYIDKHGTGGNWIALPQKA 204
10) MYCA1 submitted from scaffold 00278 (BLASTn) tacattattttcctttctttctttCTCCTTTCTGACTTGATTGACaTAGTATTAACCGATACGATAGTATAATAGAaCAAGGGATCTACCCTGTTCCCTTCTCCTTTACGGTTCAGgAATTCATCTCCATTAGTCATGAACCCCGcTTGgCCcGCCATTGAGTCACAACTCTTCTtGTACAAACTTCTTTCAACTgaTCAGTCGATAGGAAAGTCGGATGACTCAGATGCCTCTACTGTAAGTaGTGGGAggtGCATAATtCATTATACGGGGGaCCAAATCACAATTTtgAGGGAACATTCCTTCATAATTtATACAAACAAAACCTAAAATATAGTACCAATAATGAATAATTGGGAGgTGTGCCTACCCGGGCCCTCGTATCCACCCTACCTGcAGGCTGAAATATTTCCTTTGAATATGTTGATGTAGTTCcCCTCAATTTTTTATTTTTATTTTTTAGAAATATCACCGTTTGGAAAATCTCAAATAAAgAGTAGTGGGATATAAAATAAGGAATCATTTCCTCTCCATACTCAATCCCTCCATgTCTTTCCTTACTCTTAATATTCATTCATATTtCAGGGCCAAAACAATGATCCTAGCcGTTCACATCGTAGAACACaTAGAAAAATATAGGTTTGCAAAATATCAATATGATCATCCATGATAAATTTGTGAATAAGTTACCTTTTGTTTCGTAGATTGTAAGGTTAAACTGTAGAATATATTTGCCATTCATTTTGCATAACAATTTGTAACATGTTTACATTGTCATTTAAATTTGACTTTATATTTGAAACTTTATATTATTTTAATTAGTTAATTAATTTGTCTTAACAATGAATAATAAAAAAAaaTTcTAAAaGTGAaTAATATTACAATCACGTTTAAAaGTGAAAAAaTaTTACAATCAACGTTGGCAATGTTCAGCCCTAATCAATATTGCCTCCAAATTGTGGTCCCGGCACAaCGCCCAGTACGTGGAACCACAAATGAAGGCGCGTGTCATAACTTAAAGCCCATCCTTTCACTTCATCCGACACATGCTCCCACCActATATTGGGATGTGTGGTACAaAGTGATGAGGGTGCAAAAAAAGTTGCACGAAATGATTGCTAATACGGGCAATAGCTATTTCTTTGCTCTGTTGGACTGGAACTGGAaGGgATCTCAaGGgCAAGTGAGTATAACAACCTCCAACATTCACGTCAAATTTTaCAGCACAAAGTTGTAACGaGATGCAATGACTACCgCcAAACTTATACAGTCCATCCAATTTGATTCTGGAGCTGTAGGTAGGAAATAACtcAGTGACAAGATATTAAaTCATTAAAAAaGAGAAACAAGATATTAGAGCATATTAAAAAGATAAAaGAGAAAaaaaTATtAATAAAAaaGaTAAATAAATAATtATATAAAAGAAaTGAATTAAATAAAaGAATTGAATTAAAtAATTAAATGAATtAGTAaTATAAaGAAATtAATTAATATAAATAATATAAATAAAGgAGGGAAGAGGGgTAAAaGGGTAAAAGGACAACATAAAGGTGGTGGGACAACATAAAGCTTAACTGAATCAAGAATTTGACTGTTCTATGTTCAaaaAAAAAAAaaaGGgAaTTTGACTGTTCTAGGTAAAAATAaTGTCTTTAAGAGCTCCCAAACAAGAGAACTTATTATGAAGCTGATAAATAGTTGAATTTGCAGTCTGCCATTGCAATTCCTCCTCAACCCACCaTAAAAGCAAGTGGGTCTTCTTCTTTTGAGCTTGAGAGCAGGATCTGATTGCAGAAATGGAAGATTAAGAAGAAACGCATCAAATCCCAGAGTGAGTTTCTTATGTGCTCTTTAACAAGATAGTCTTCTCTGAATAATCTACTTTCTTCCTATTTACTTCCCCCCCcAAAAAAAaTCTACTTTCTTCCCTCCAAATGACTTTTTCTTGGTTACAGCCTTTTCCCCTTTTCACTGTGGTTGATGGCTTTTTCTAACCTCAACATGACAACATCAGGTGCTTATGAAAACCCTGATCTTGATTTTTTATCTTATACAAAAGCTGAACTAAAGCTACTTCAGAAATCCTAAAGTTCTCCTTTAGAATGGCTTTTCTAGTTTTTGCTTTGCATCTTTTGACTAACTGAGTAATGGGTTTTCTTAAAATTTGGTGACtaGAGTTTTGAGGGTTATGTGTTGGGgAATGGCTAAAGGGGCACAAAACCAACAAGGGGTGCCAGAAAATCTGaGAAGGCAGCTTGCTGTTGCTGTTAGGAGTGTGCAGTGGAGCTATGGAATTTTCTGGTCATTGTCAACCAAACAAGAAGGGTATGGTTTACCTTTTTACTATTTTATTTATCTTCATATTTTTATGGTATGTCCTTAAACATTCTATTTCTTCAGTTTCTGTTGCTGGTTTTGCATAAATTTCCAGATCTGGGAAGAATAACGTCTAGTTATGCTCATTATAAATGCTTCCCATTCCATAAAAAAaTTGATTTCCCTGCTCATTTATGGCCTCTTTCCTGCAAATGTTAATACGCCTGCTGATTTATGGCCTCTTTCCCTGCCCAATATTTATGTTCATgTTTGCCTAGTATGTCATTACATAATTACATTTTCTATTTCTTCAGTTCATGTTGCTGTTTTCTTCATTATTTTTTGATAAAATAAAATAAAACAAAATTTCGTTGagAAAGCGACGAACGAGACCAAAGCTTCCCTTACAAAGGGTGAAATGCTGAAAGGGAAgAGATTGAAAGAAAAGTAGCTTCAAAGTTCAAACCTAACACAGGATAATGCTTTtCTAGGGAAATCAACAATTTTCATGGGAAAGCCATTGCTTATAGCAAAGTACACCTACATGCCTTGGTACATTGAAATTCCTCATGCTTAACTGATAAAaTATGTTCCTTGTTATTTTGTTAAGGGTATTGGAGTGGGGTGATGGGTACTACAATGGGGACATAAAGACGAGGAAGACAGTGCCGGCCATGGAACTTAAACCAGATAAAATGGGTTTGCAGAGGAGTGAGCAACTGAGACAGCTTTACGAGTGTCTCTTAGAGGGTGAAGAAAGTGATCAACAAGTGCCTTCTGCCGCACTGTCTCCAGATGATCTCACAGATGCAGAGTGGTATTACTTGGTTTGCATGTCCTTTGTGTTCAACCCCGGCCAAGGGTACAAGCTTTCTTCTGGACATTTTCATCTTTCTCTGTGgCTGTGAAAACTTaGTTACACTGAAAACTAAACAGAAAAAACCGAGATGTGACCGTTCAAATCTAAACAGAAAACTTAAAGTTTTAGAGTTTCTGAACTCTGCAGAAATTCCTGTGTCCATTTTTGTTtATGAACAAGAAGTATAGTAACTTGCAAATATtATGGAGTAACCGATATTGGATCATTTGTGTGTGTTCATTAAGATAATGATCAGTTTTTGAGGAAATTGCTGTGTAGTTTGCCAGgAaGGGCATTGGCAAATGGTCAAGCTATCTGGTTATGCAATGCTCACAGTGCTGATAGCAAAGTGTTTtCTCGctCTCTTCTAGCAAAGGTCcGTTCTTCTGGGGCTGTGCTCCGTTTTAGATTTCGTGCCAATTTTAGCTTGTTTACTAGACAAGTTTtACGATATtCAGCTCAATATACTCTCTACTAGTTAACTAACAGTGATGCTATAATTTTCTTCCTTTCTCAAATGGTTGGACTTGCGCACGAATCAACCTCAGAGTGCATCTATTCAGGTAAGCCGTTATTTTTATTAATTGGTACGCAAGTTTGCAAGCACACACATTCACAGGCACACGCACATAAGGACGTTGGTGGCCCCATATAATAGATCAAACAAGCAGTGAAGAATTTGTTTCCGTGGAGAACGTTTTTTTAGTAATTTTACTTGCAAAAGCTTTTCATAAACGAATCTTGTTGCATATACCGGTAGTCTACTACAATGACCATTGTTTtATTGCAGACTGTAATTTGTTTTCCCTATTTGGGTGGAGTCATTGAGCTAGGCGTGACGGAGCTGGTAATTTCTGTTCTATTGCTGGATTTACCACTATTTTTGTATTGAAGATGCATCAGATGCTATACGAATTATCCCAGAAGTAACTGGTTCTGCTATTCTTTTGTTTTCcTAAGGTATTGGAAGACCCTAGTCTCATTCAGCACATCAAGACTTCTCTATTAGAGTTTTCAAAGCCCGTTTGCTCTGAGAAATCTTCTTCTGGTCCTCCCCACAGAGACGATGACGCTGACTTCATGTATGCCAAGGTTGATCGTGAGCTGGTAAATACAATTTCGTTGGAGAGTTTCTATTCCCCAACTGAAGATATGAGATTTGATCGGGAGGGACTAAATGACTTTGAAAAAAATATCCAGGCCGATTTCACTATGGGTTCTCCTGACGAATGTTCGAATGGGTGTGAACACAATCACCAGACAGAAGACTCCTTCCTCCTTGATAATGTAACTTCTCAAATGGAAGATGATTTTAGCAACTGCGTTCAAGTTTCCATGAATTCTAGTGATTGCATATCTCAGGTCgTTGTGAACCAAGAAAAGTTTATTTCTCCTCCCAAGGGTGACAATAGTAAATTGAAAGAACTTCAAGAAGGGAATGATACAAAATTTGGATCCTTGGACATTGGAACTGATGATGACTTGCACTACAAAAGAACTCTATCTGCTGTTCTGATAAGAAATTCTCATAGGTTACTTGATAACCTTTGCTTCCATAGGTGGGATTatAAGTCTAGCTTTGTGAGTTGGAAGAGAGGAGAAATCAGAAATGGCCATAGACCACAGGTGCCGCAGCAGATGTTAAAGAAGATATTAGTTGATGTCCCTcTAATGCATtGTTTCTCACTCAAGTCTCAACAAGGAAAtgTGGGGAAATTTtGGTTGTTAAAACAAGAAAGTTATGTGCAACATGATTTGCCGGATGATGGAATAGAGAATGAGAAGTTTTtGGtCCTCAAATCGATGGTTCCTCCTTCTGTTAGCAAGGTAATTCAACTGCCATCTATTGGAAAATATGATCGAATGACTCAGAATTAAATATTTCTTTACACTTCTTGCTCTCAAGACTTGCTCATTTCTATGTAGAAAATCAGGATGGCAGAAGGCATTGACTTAATCCCCACCCTCAAAACAGAAGGATTCGAATTTTCTGAAAAACAACTTTTCGATGTtGCTAATTTTAGTCACTAAAAGTAGATTTTCAAGAATAACATAATTTTCTTGGGGTGATGGTATACTATATTTGaGTCCTCAGCATGTCACATTACCTTCACTAATCAGAGGGAACATATAAACAACATGGAAAAACGCACAGCAAAAAAGTAGGAAACGGGAAAATCCTAATCTTATTCATCATGCTTTGCAGGTTGACAAAGCAGCAATACTTGGTGACACTATAGAATACTTGAAAGAACTTGAGGGAAGGGTAGGAGAACTTGAATCTTGCATAGATTTAGCGGAGCATGAAGCTAGAAGAAAcTACCCAGATGAAGTAGAGCAGAAATCTGAAAACAATGAAAACAGAAGGATTGGAAATAGCAATAAGCCTTGGATAAACAAGAGGAAGGCCTGTGATATTGATGAAACAGACCCTGAGCTCAGCAGGGTTGTTCCTAAAGATGGGCCACTGTTTGATATGAAAGTAAaGATAAAAGAGCAGGAAGTCCTGATTGAGATGAGATGCCCTTCcAGGGAaTATTTGTtGCTTGATATCATTGAAGCTCTGAaCAaTCTcAACTTAGACGCTCACTCTGTTCAGTCaTCAaCCaTTGACGGCATTCtCACTGTAACCCTTcAGTCTAAGGTCTACTTCTTTCCTAaATtGTTGCaTATTTAGGtCTAAATTTGTTGAAGATATAATTAATAAAGTTGCCCcTCTCTCTAACAGCTTAAGCTTTTAGATAAGTTGGTGGTTAACAATTCAATaTGGTATCAGAGCAGGCAATAGATCCTGTgTTCGAATCTCACTGCCtaCCTGATAATTAAATACTTGCGTGTGTTTAGCCCGCTTATGGGGgAgAGCATGGCTACACGTgAGGGGTCgTGTTGAaGATATAAATAATGAAGTTGTCCCTCTCTTTAACAAcTTAAGCTTTTAGATGAGTTGGTGGTTAACAATTCAATAAAATTTTAGAGACATTAATCACTTTCCTATTTCATAGTTTTGGAAAAAaTGTACTTATACTAGAAATTTTGTGCTACATATGCACTTTCATCGTTTCTGAGATGCTAGTGATTCTTTAAAAACTTGATTTCAGTTTCGAGGAGCAGCAGTTGCATCAGCACGGATGATCAAACAAGCGCTTTCAACTGTTGCCGGAAAGTGTTGATAAACTCAAGTTTGCATATTAATGACTTCTGTCTTAGGATTTGCTTCAAAACGGTGTTGTGTAATATGTATCATTGCAGTCCTTTCAGTCTTTGAGTTTTCTGGTCTCTTAACAATCACTTCTCATGGTTTACTGTCTAACCTAGGAGCATAAATGTGGATGTAATTTGGCTACTATCTGCACCATGTTTCAGTTTCATGTCAATGAACTACTTCGATTTTCAGCCTTGCTAGTATTTCAATCTACTCTGGCTGATGCAATAGGAGTTCTTTGGCACATGAAGAAAGGGAAATGGTTAAGGAGAGCTAAATCTATTATGATTGTAGTTTTACAATTATGACTTCAGTTCAAACAGTTACTGAGTCTCCATTAAATTGAGATGGTAATTAACACCCTGGGGATGATGCAAGCTGGTAGTCACCTGCCAACCATGGGTCTCAGGTAGTCATGGGTTTGACGTCCCCAAGTACTAGACGGCGAAGTTAGTTCTTCAAATTAGTCGGCCCACTGAGCCGCATATCGAGTTATAAAACAAATTGAGATGGTAATTTGAGTTGATAACCAATGCCTAAATCTGTCCTCAAGATTCTGAGCATTAAGACGCAACTTTGTACTGTTAGCAAGATTTGGCATGCATTAGGAGTTAAACATTCAAGTTGCTTTCCACACCCCCTACCTCTTTCTGAATTAACTATTTGATCCATCATTTACTAACTATTTTAACCTTTGGGAGTTCTTGAAATTAATACCCGAGTTTCCACTATTGACTAAACGGATTTTCGGTATTTGCTTCAAAAaAaTTCACTGTACAAGACAAGTCATTTCTCCTAACTAAATGGACTAAATGGTGTTTGTAGTGGGAACGAGTCGTCTTTTGTCATCAGGCTGAAGATAAAAAATATATATATATAGCGTGAACAAATCACCCTTAGTTGGGAAATCTTTAAGCTGAAAAAaAACTGCATCATGCATAACAGGTGCAAACAGCTTATTGTGATTTCTACTAATTGTTCAAGCCATTATAGTGTCTTCATAAAAGGGCACCAACTTTTCATCTTGTCCACCAAAGCTATTAGCATGTGATATGTGCTTAAAGTTCCACAGCATAGCTGTGTTTGGCTAATCCCTTGCAGGGACATGTTTTCATGCATTTTACACAGATTAAAGTGGGCAACGTACCTTATTAGGGACTAAAAAAaGAAAaTGTTGCTACCTATATATAGTAGATTAAATCAAAAGTGGGAACATACCTTATTAGACATGATTATGATGGAATTTGGAATTGCACTGTACCAAAGTTTTGTTTCCAACTACTACCAACCGGTCAAAGCCTCCCAGTCTCAATTGCTGCATTTTtCAAATTACTtGTTTtCCCCATCAATTACCCACATACATATAACTCACCACATATTCACTCAATTGTGTTTGGATCATGAATTtCCGGCAAAATATGAAAAGCGAAGAAAATAACGAAGTGAATAAGCTAAATTTATTTCGTCAACTACCACTATTATACCTTTCGTAGATCCATAATCCAAACACAGCCAAAGTATGACAACCCAGTGATTCCAAGAAACCACAAAAGAGTCTTATTCTACAACAGATGATTGATAAGAACTTCTTCTACCATGAACAATGGCTTCTATTTACATCCACTACATTTGAAATCTAATACCAGCAAAATCTCTgACAGTAAAGCCAATTTTCTCAAACTTTCCCTTCTCATTGCTCTCTCTGAATCTCAAATAATCCGAGCAAACGAATGGCTTGTAAATCCTCGAATTTCCCTCTCCTACCACTTCATCAGGTGCCAAGATCACCTTATCATTCTCAAAGCACCAAAAGAAAGCAAGCGAAAACCGGTTCAGTGGTTTTTtCAAAACAACTCTGTGTTCTGATGACCTTAACTTCTCATTGCTCCAAGCTTGAAGCAAATCACCAATATTGACcACAAGGGTCCCCTCACATGGACTAaTGTCAATCCAATGGCCTTGATTTGATCTCACCTGGAGCCCACCCATTTCATCTTGATACAAAATTGTTAGACAACTCATATCAGTATGCATCCCAAGACCCTCAACCTCTTCTTCCAAACTCTTTGGAGCTGAGTAGTTAATTATCCTCAAATAACCATGGCAGTTTTTGTACTCAGAATCATAATACTTTCTTTCAACATCATCACCCAAGGTCATCAACACAATCTTCACAATTTTCTTCGATAAATCTGTCATTGCGCTTCCATATtGTCGCAATATTTCACTAACCAAAAGAACAAAAAAaGGAAGAAAGAAAACTGTCAACGAAATTTGAACGGAAACAGATGAAGGGATGTTAGGTTGGACTTTTCAATTTATGCACAAAGTGTCTGCTCTTTGAAACTTTTACCACTGAAATAGCAGCCCCTTGTACCCTTCAAATATTGAGGGACTCAAGGGACGTTAGGTTGGACTTTTCAGAAGCTAAGAAATATAATTGACGAGCTGAAAAAACTTCGCTGAAGACGAAAAGATTCTGAACAATTTTGTACTTAAACATCCCCAAAGGAGTTTTAGCCAGATAAGGTGAGTACGTAGATAGACAATAGCATTACCTGAACTCAGAATAGTGCTTGTCAAAGAGAACATCTGCTGAACTCTGAGCAGAGCCGGTGAAGTCTGGTCCAGAAACTCGGAGGCTCTCAAAAAAaGGAGATGCTATGTAATGAGGAGTGTAGGTTTTGATAGACGAGAAAGGACCGAGTTTGATTTtCGTCTCATCAGGAAGACTGAAGAGGAATTCTGAAAGTAAGCGGATTTTATCATAAAGGTCTTTCGAAATCCCATGATTGGTGATGTGGAAGAAACCCAACTCTTTGCAGGCTTCAGAAaGAGAGGACAAAGAACTAGAACATGGCTGCAATGGCTGAGAGATGTCTAaTATGGGtAGTTGTATGCacaCCTTGGACACCATTTTctATATACAGGAAAGGCTTGGTTTgTCCAtcTAGGTTGGGGCTATTATATATAAATCAACTGATAAAACAACTTTtGACACTACCATAATATtATtATACAGAGGCCAGAGTAtGGTAGAAaTAGAGATTCACTGTATagCATAGTTGCTTAGGAAGTCTTtGTAAaCAAACTTTCATGAGACTTGTGAGATTAAACATTAAAGGCGTAATTATCTTCGCTTGCCATAAAATGGTATAATAGTAGCCAATTTTTCGCCATTTGTACCTCAACCGTTCcTACTTTCGTTTtCAACTAGCGTACATTCAAAGAGGAAAGAAGAGGGGAAAGGCATCCCATTATTGGTGATGTGGAAGAAACCCAATTCTTTGCAGGCTTCAGAAAGAGAGGACAAAGAACTAGAAGATGGTTACAATGGCTGAGAGATGTCTATTATGGGTTAgTTGTATGCATGCCTTGGACAACATTTtCCTATGTACaGAAAACGCTTGGTTtGTCCACCTAGGTTtGGgCCATCATATATCCTAtATGGCTATTCTAGTCTTTtGAAAATGAAAGAGagaTATATGAaTGTcTATGAATCAACTGTCCATTTAACCCTcTTTTTtAGAACCctATGATCATTCAAATACtGTCATCAAGCATGTACAGTTCagTAACTCCCAAACTACCATTAAAACTATAAGGACCTTTAGGTAGTGTAACCGACAAGGATTGAGCTCTCTTTCGATGCTCTCAGATTGATCTAGTgaGGAATAGAATTGAGTAtGATGTACATTTCATATGTGCTATcGATATCCTCATCATATATGCAAAAAATACTGtATGTACGACGATAATGTTGtACGTTGATGTCTATtGAGCTTTTACCaTCATAAGAGTAcAATTTTACATGAAATATCCTGTGATAAACAACTTTTGACAGTACCATAATATTATTAATAGAGGtCAGAGTATGGTAGAATAGGGATTCACCGTATAGCATAGTTGCTTAGGAATTCTTTGCAAACAAAACTTTCATAAGAcTtGTGAGACTGAACATCAAAGGCGTAATTGTCTTCACGTGCGATAACAAGGGTATAAAAGTaGCCAAaTTTtCGCTGTTTGTACCtcAACCTTTTCTACTTtCCTTTtCAACTGGTgtACATTCAAAGCGGAAAAAAGaGAGGAAAGGCATCCAATGCAAAATaCCCGAAGTTATCaTTTTTCAagCAATGCACAGctCTAAACTGAAGTATTAACTTTGCCTAGTCCATGTCAAAACATGAACCAACCCAACACCCCTtATtcGGTGCAGCTTAGGTAATTATTGAGCCCAGAAGAAAGCTAaTGGACGTTAGAGTCCCCCACAAAAAGGCAGATAAGGGGGGAAAAAGAAAACAACATTGGAGACTAAaCAAATCTCAATGGGTAACACTCTTATTAGACAAAACTGCTGTAAAAAGTTCATTAATGAGTTCTGGAACATTtACACACCAACGCCACAACAACCCGAATTACCCGGTTTTCTAATGGAAACAGACAGAGTAATGATTTGATGCATTTTTtGAtGAAAAaTtCTACAATCAGCCCAATGGGCTATCGTTAAGGTTTTAAAAGTGGGGACTTTTCCGCGTAAGAATCTTCAAAAAGTGGGAACTCTTGCACGCAAGAGTTAACTCTTTCATGCAAAGGTCTTACCTTATTGTCAATCTAGTGGGGACAGAAGGATTGTTTTTTTtATTtGCGGCCACTACACGTTACTCGGCCAAATaTATAGGAAAATATGACCTGCTCCTTTTCTAAATGCAGGGCACATTTTTtGTTCTGAAAAGGAATCTGcGGAGGTAGGTGATTTGGCTCcCATATAACTGTGTAGGAAAGGTGGTTGACAGGTTGAGTAGTTtGTATCTTTCTTCCGGTATTGAGTACCGACTCAACACGGGAAAACTTAAGAcgTCTAGACATAGTAACCATTGACAAACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGACCGTTGGTgTtGTTGGTTCGAAAAaCCGCACCCCCTTAAATCTTGGAAGgAGTTCAGTGAATCATATtGTTAGCCGTTTTGGAAGAGTGTTTGAAGcGTTGGAAATTTACGAAATTTCGGGTGAATAAAATCGGGACATTTGGAGGGTCTCACGAGAAAACTGAAAAATCCTCTCCAGATTCCTTGAATCTGGACAAGGCAAAAGTTATGGGGCTGGTCCCATGATGTGTGGTAACGTCTCAAAATTATTTCAGCGGTTTCAAAATTAATTCTCACCTAGCAACTCAAGGCTTTCTCCTTGTGTATATTATGGAATCCCGTTTCTATAGCCTGGCCCAGGACCATAAATTCGAATTCCATAAGACATATTTTGTTTTTCGTTCATTATGTATTTATGTGAGAGACACACGCAAGTTTAGTTCGCCACGGCTGTTTTCATTGGTGCACTGTGATTTTAGCCGTTTCATTCTGGGATGTAGACTTGTTGGGTATGCAGACAACTTTCAACAATAGCCCGGGGTGAAGGAGGCAACGCCACCACGGCAGTTGGCACAAAAGTTTGTGCCGCAATCCATGTGCAACCCTAAGAGTAcACGTATTGCGGCGAAAACGGTATTTTGTAATTGGGCTAGAGCAATTTCGGCCACCTATTGCAATTGCTTTGTAATTAGGATTCGAGTGTTTTCAGATTAAACACGAGATGAgAaGAGAACTTTTTtGAGAGAGAAGAGAGGACTTGTAGAGTTATACTCCATATTAGAACCCcAAAACGGGTAAGGGTTTGGGTAAAGAGTAAAGATTGATTTTCGATAATTTtACCCAAATTTtGAGTTTGGATGTTAAAATGGGTCAAAATTGGAGATGCTTTTAGAGATACTGGATTTTGATTTTTAAATTTATCACTTGTTGTTTCTCCACAAGGAATTGATTTTGCACTCAATTTTTTTttATAGATGCaCTCCAAAACTTGTCTTTTATTATTtCAAaTTACATGTAACAAATTTtCAAAGCACACTTAAAacAaGTGTACTAGTTCTTTTGTTcGTTTAAAaGGgAAAAAAAAAaTaCTACAGTAATATTCAATGGAATaTTAGTCAATTCGGTTTCCCACCGGTGCAAACCAGTATTTGAGCCGGAACCTACTGCTAAACACGCTTGAGAGATCAACGACGTCGGGTGGGTTAGGGAAAAAAAAAaCCTCCAAtACGTCGCAGAAGGGACCTTGTTTGAATTACGGCGGGACTTGCAACTCTCTCTCTCTCTCATGAGCTTTCTCTGTCGTCTATCGTTTTCCTCTATATATACATAGATGTGCCCTCTTGTTCGGAAAGCATACAACCTGTTCGAAGAAATAgCCCAAAGGTGCCCTTTTGTGCGAAACTCTGTACGTTGTTTATCAAACTGTGCGTTGAGTCAAACCCATTCCTATGAAGACCCAATTCAATTTTCTTATGAAACTCAAAATGTAATATCTTGGACCTCAAAAATCTCCGCTTTAGTGAGGAGAGAGAAGCCCCGAGAGGCCATTGGGTTGTTCAAAACCATGCTTGTGAAAGAGCAAAGGCCAAATTATGTGACAATTCTGAGCATCATACGCGCTGTTGGCAGTTTGGGTTCTGAAAATATGACCCAGGCAATTCATGGGTTGTTGTTCAAAATGGGGTTTGAATCAGAAATTCCAGTCGTAACCGCCCTTTTGGGTGTTTACTCTATGTGGGATGTTGAAGTTGTATGGAAGCTGTTTGATCAAATACCCAATAGGGATATGGTTTtATGGAATGCAATGGTTTCGGTGTGCGCgAaGAGCGGGCAATACAGGGAGGCGTTTGAGATTTTTAGAGAAATGCAGTACGATGGTGTTAAACCAAAAGATGCCAGCATTGTAAGCATACTGCCTGCTTGTGCTGCTCTTGCTAATTTGTCCTTGGGCAGACAAATACATGGTTTCTCTGTAAGAAGTTTATGTTGTTCACTTACAAATGTTCAAAACTCACTTGTAGATATGTATGCGAAATGTGGAAATCTTGAGACCTCTATTCAAATATTCAATAGAATTGAAAAGAAGGATCTTGTTTCTTGGAGGACCATGCTTCATGGGTGTATCGCCAATGATTGCCCTCGAACGGCACTGAATATGGTCTCTAGAATGCAATCTAATTGCTTTAGACCAGATGAACTTTTGATCCTTGATGCAATTGGAGTCTCTACACAAGAAGAAAAACTTAGTTTTGGGCTGGGATTTCATGGTTATATCGTAAAAaGTGGTTTCTTGGATTTTGTCTCGGTTGTGACTGCACTTCTCCAAaTGTATGCTAGGTTTGGTGAATTTGGTTCAGCTGGGGTTTTATTTAATCATATCACTCAGAAAGATGTTATTGCGTGGAGTTCCATGgTATCGGCATATGCGCAGGGTGGACATCCTTtGAATGCTTTAGAAACTTTCAAAAAGATGCAATCAACAGATGAGAAGCCGAATGAGATCACTCTAGTCAGTGTATTACAAGCTTGCTCTtCTTTGGGAGTCCAAGAACTTGCGGAGAGTATCCATGCGCATGTGACTAAAGCTGGCTACTCTTCCAACACAATATTACTACCAGCATTAATTGATATATATTGCAAATTCGGAAGGATAAGGCAAGGAAAGGCACTTTTTGATGAAATTCTTATCAAAGATCTTGTCTGTTGGAGCTCAATGATCAATGGTTATGCGATAAATGGGTGCGGAATTGAGGCTCTGGAGACCTTTTCAGAAATGTTGAAGTTTGGAATAAAGCCCAATGAAGTTGTCTTCATTTCTCTTCTATCTGCTTGCAGTCATTGTGGGCTAGAAAATGAAGGCTGGACATGGTTTTATGCAATGGAGAAGGTATATGGTATTACTCCAAAACTTGCACActATGCTTGCATGGTGGATCTGCTTAGTCGTCAAGGACATgTAGAAGAAGCCCTTGAATTTGTGAAAAGTATGCCTATTGAGCCTGATAAAaGAATTTGGGGTGCTTTACTTGCTGGTTGTAGATCAATTCATGGCtCTATTGAGATAGCAGAATTTGCGGTGGAACGGCTCATTGATTTGGACCCGGAAAaTACTAGCTGTTACGTGGTTTTGTCAAATTTGTATGCAGAGCAGTGTAGATGGGAAGACGTGGAGAGACTGCGGGAACTGGTAGACAATAAGGGATTGAGGAAGGAAATAGGATATAGCATTGTTGAAGCCAATTCATAGCATGCATATTGTGAGATTGGTTAGCTGTGTTCTCTCAACAGGTTTCTTCTTGTTTTTtGTTtGACTATCTGTAGAATGCTGAAAATAAGTTGCTGGATATATCTCTTACTCAGAGATCATACATCCATTTTCAATCTCACCTTTTGGTTTGTCCGTACATCACACTACATACCTTAGACAGAACTTCTTTGAATTTTCCAGTAACGTGAATTTAAATAGGCGTCTGTGAATTTAAATAGCcaTCTATGTATTTATTGTTGCGGAGTTACATTTAGTAGCGGCTTAAGTAAAGAATTTATTGCTCATTGAAAGCAGTACTGTATGCTTCCTTTTATATCCTCCATTAATGCGTATTTCACTAAGTTAACTTTCCAGACCCTTGTTACTAGTCCAATAAATTCTCGAGATCTTTTCCATATAAGCTTTGATTTATATCCCGTTCTGATGAAAGAAGGAATCCTTGAAGTACTTCTTGTCAGCGCTAAAGACATTAGAAACACCAATGTTATCGGTACTCTGTCTCTCTCgCAGCTAATGTCTAAagAGGCCATGTGTATATAATATTTACACGGATTATAGAAAGCACTTTGTTTCTTTGTGTGGCATTTTTTGCTTTCTCTTTTGCATTAATTGGAGCTTGTTAATTGTTCGAACTCAACCTCGTGTTTAACAGGGAGACCAGCATATTATGTGCTCATTGAATGTGGAACTCAAGTGGAAAGAACCAAAACTACAACAGGTCAAATTCTTGGTGATAGTTCATCCATCTTCCTAGCTCTCATCCTAATATGCTTTCCCTAACCTAATTACAGAGTACATTGGTGCAGGTAATCACAAAGAAATATGCTGGAATGAAAAGTTTGCGTTTGAATTTCCCTCTTTCGAATGGGAAAACTTGACTCACCTCAAATTTAAAGTCATGGCTGAGGAATATTTGTTTGAAGGAGGATTTGTTGGTGAaaCCATGTAAGCAGTTTTAACTCAAAACATTATTCTTGTTCAGTTTTCTTTGTTAATTACCAGCCATATAATGCTTTCATTTTCTTTGTTAAACAAACAATTTTGTACTTTCTGCTAGGaTAAATaTCaCAAATCTTCTAGAGCTTCTTTTAtGTcAAACCCaGCAAGGAACTTGCTCTCTCAAACCATCTTCTACCCTAGTAGTATTGAATATTGATCACACGAAGTTAAGAAAAAaGGGTTTAATTTCTGCATtAATTTTTtGTTTTTtGAAATCTTGTGTATGCATTAAGACATTTACTTTGGCATTGGAAACCTTAtGAGTTATGAAGGAATGAATATGTCTTAGAGGAACCGTTGTCTCTCCCTCAATTTCTGTATTTCTATAGGTTAAACTCCTCGTGATGATTATGAACAATGTGATTTGAAGCTTTGAGAGCTTTTAACTAGTAAAGCAGTTCATGAAATAATGGAAGTACTAGCTATAATATCTCcTCATTTGCGTATACATACATTTTGTTTTtGTTGAAATAATTTCTGTATCCCAGATTCtcGGGgCAAATTAACTATCTAGATATTG
>CF810540 Length = 633
Score = 69.9 bits (35), Expect = 4e-11 Identities = 66/75 (88%), Gaps = 1/75 (1%) Strand = Plus / Plus
Query: 5863 tgttgaagatataattaataaagttgcccctctctctaacagcttaagcttttagataag 5922
|||| ||||||||| |||||||||| |||| | |||||||| |||||||||||||| ||
Sbjct: 392 tgttaaagatataaataataaagttatccctttatctaacag-ttaagcttttagatgag 450
Query: 5923 ttggtggttaacaat 5937
||| |||||||||||
Sbjct: 451 ttgatggttaacaat 465
11B) MYCA1 scaffold 00554
Submitted from SSR primer area
GAAAACTACtAaGAAACATTGTCACAAATGCAGAGGAACACAAACTcTTTCCAAACCAAATGAAGAAACTGAGTTACGTACGCCCAATGTAAACATTATGACTTTTTACtaCTAACCCTAGTGCAAATTATTGCACTATTGTTGCATTATGAAATTCTAAACTATCACAAGAATAATGAAGGGGTTTtATAGGCTAACCcTAacAACCTTAGCAAAATCCAAATAACCCAAAAGGACTAAAATACCCTGAACGTAAAAcaaaaaTAACGATAACCTTACAGAAACCcTAATGACACAAAAAGACTAAACTACCAtGAACATAAACAATAATCTGAAACCCTAAAttACACTCCCCCCCCcTCCTCcTtCAAACGCAGGATGCGAAGCCGGAGAGCATTGTGAGTTTGCCAAGGGATTAACAACAGCGAATCAACTGCTGTGATGGTGCTGAACAATGCAAAGCaGTACAAAACTAGAATtTGCGAATAGGAAGAAGAACTGATGCCGAATAGATGCAAATAGGGGAAACCAATGCCAAACAGATACCAACCAGATGTCAAATTGCtGATGCAAACAATACCAAGAGAGAGAAGAGAACAGAAgCAAAGTGTCAGATCAATTAAGATGCAAAGATCGACAGAGgTGTAGGGAGGAAGAACAGATGCAgAGAGATGCAAAAACTGATGCAGATTTAAGAGGTTCAAAAGTTTAGAGGTTCAAAGGTCCAACATAGCAGAATATTGCAGAATCAGAAGCCCAAGTAAGAGCGCAAAATACCATAGTGGTTGTAGAAGcGGAGGTCGATATAATGTGCACCAGTCAATCGAAAGAAACCCACTCAATTGATGCAATCTGTCGATTCCAAGTGCATTAGGGAgaGACCCAGTCAACGTCGTGGACCAACCCAATCAGTTTAGAAGTCAATCAGAAgTtACCCAGTAAATTTAGGTAGAACTACCCAAAAGATTATGAACATGATCTGAAGTCATCGTGGCCaGaCTGGAGATTGGTTCGCCCAAGGTCGTCGGAGCAAGTCAcTGGGTGGgAGGTGGCTGCTGCTGCTATTGTGTTGTTGTAcGTGATGTTGCTGCTGGTGCGATGAACAGTTCATgTGAATGCCCCGcGAATGGTGGCAAGCCTTAGGcTgCCcGCCGGCGAGtGAATATCCGACAtgCCTAGATCTTATTGCTCTGATACCATGTGACTTTGCAAAGAaGGAAAACAAAACTAACGTATTATtGCaTTATGAAAGTCTAAACTATTACAAGAATAATGAAGGGTTTtATAGTCTAACCcTAAAAaCCTTAGCAAAaCccTAATagCCCAAAaGAAAaTACCTGGACATAAAAGATAAATAAcGATAATCTTActGAAACCCTAATGACACAAAAAGACTAAACTAcAATGAAAATAAACAATAAACAAAAAGACTAAATCAAAGGTTGGTAACTTTGCATGTAAGGGACAACCCACAACTCTACTAAAAAaGGGGGACAAACCATAGATTTCTTTTCAATAACAGCTTtCTTGGAGAGAGAGAGAGAGAaGCATACcTGTAATAAtCCTTTTAGGTCCAAGCTCCCTCACAAAATCAAGTAGTCTAGACAATGCATCAGGAGTACGAGCATAATCCAcAATCACCCCAAATGCTTGTCCCTCATCTATCAACTCACACCTACCAGGAACTGCATCAACCTCTtCAATCCcTCTAACAATATCCTCCAAAGGTGCTCCAACCGCAATTCCAACTGCAaCAGCTGCAAGAATGTTGTATACATTATGTCTGCCAAGCAACCCCGAAGAAATCTCCAATAAACCGTCGGGCGTTTTTACCAAGACCTGGGTCTCAAATAGAGATAGTTCAAATTTCAAAGAATGAACGTCAGCatCTTTATTCTCCACTGCATACGTCACAACAGGCACATCTGGGTTgCCTTGTGCAATAAAGGAaGCTGCATTCGGATCATCAAaGTTGACGATTTTCCTGTGTCTTTctGGGTCCACCATTCTTGAAAACaGCTTGACCTTAGCATCCCTATaCTCTTCCTCagaCCcTTCAAAaTCGTAACGgtCCCtGTCAAATTTGTGAAAACGgCAATGTCAAAGTCAACCTCATTGCACCTCCCTAATGCTAGTCCATGTGACGAAGCCTCCATCACAACTGCTTCTGTCCCATTATGCACCATTTTCGCCagCAACTTCTGAACCAAAACAGCATCTGGAGtcGTACTTGGCGACTGCAACTTaTTATCTCCATGAATATAATACGCCACGGAGCTGAACATCCCAGTTCTCAATCCCATGGCTTCATACAACCCTTTAATCAAATACGCGGTGGTAGTCTTCCCATTTGTACCAGTAATCCCTATCACGGACATTTTCGTAgAGGGGTAaCCAAAAAAAGCTGCCGCCAATGCTGGgAGCACCGAACTTGTGTCTTCCACAATAACCACAGCCTTACAACCTAATGTCTCCTCTAAATCTATCTCTTTACTAGTTACCACAGCAACTGCACCTCTCTTGTATGCCTCCGTCAGATACAAATGCCCATCAGTTTCCCTtCcGACACAACACACAAaCAAATCACCAGCCTCAACAAACCTCGAATCATGTTGAATCCCACTAATCTCTACCTCTAAATTACCATAAACTGAAACAGGTACTACTTTACTCTCATCTAAAAGCTCAGCTAATGTCATATAAAACGTGGGGTCAACAATCCTAAACTTGTGACTTTTATCAATCTTATCGAAATCAATATCGAACGATGAATCAGAACTCAAATCAGACTTaCCCAAATCAGAACTCACCTCATTCTCCAATTTCTCACCCTCTTCATCATCACCCGCATCTGCCGGAATCACAGTGAGCCCTCGCAGCTCCTCAATTTCCTCCAAATCAACCACTTCTTCAGGCTCCAGCTCATCAATACCCTCATCCACTTCCTCAACCTTCTTCTCTTTCCTAGGGTTAGGTTTCAACAAaCCCTTTTCAACAAACTCTTTCCTTGACAAAGCAATAGCCTTATCAATATCAGCAAACACATCATCCCCCGAATCTGTTGGAAAAATATTTTAGAATTTCTGTAAACcAAAAAAaCCAGGGACAACAaTACAActATAGCTACCATAAACATTTGTGGCTACGGGAGTTACATTTGAAATTTGTAACTCTTTTACAACTTTCAATTAGCAAATCATTACAATTAAGTACAATTACAAAGCTATCACTGTTGTAAAATCAAATAATAGGTACCATGAAAATAAGAGAGAGTTCACTCTTGCCTTGGGcgAGGATAACTTTatCCACATTCGGCGGGgTTTTGTGGGTTCCGATTCGGTAATCAGAACCATTcATTTCGTATGACTTGATTTATCCACATTCGgCGGGGTTTTgTGGGTTCcGATTCgTTAATCAGAACCATTTATTTCGTATTACTTGATTTATTAGGAGACcaTGTGAAAaTTCAGCTTAATCATAtAGTTGTAAGCACTCAaTCCAAAATTttAaTTTTtCTCCATCCagTTCTAAAGCTAaGGAATTaGACTTtCTATGCTCAaTATctAATTGATCTAAATTTTTTTGCGTTGTCTCCTAACAAATCAATTCATCCAAAaTAAGCAGTTCCAATTCCCATGGGTAAAATTACACTAGCTCTGTTGAACAGAATTGCTCGCTACAAACAATCAAATAAAATTCAAATCACAAAAAATCTAACAGAATCACCACCTCGGTCTTCGAACCCGGGATCGTCGGGTGCGTCCTCCTCGTCGGCAATTGCATTGAGAAAAATTGATTGCTCCTTTTCAGACCGCTCTTCTTGGATCTTTCTTGCTTTATCAGCATGGCGCTTGACTTGCTGGAACCTGGATACCCCGTGCATGGAGTCTTCTTCCGCTTCGGGCGGGTCATCGTCGGATGGGTCCGGTTGGAATACGCCGTcGGGgCCGATTGCGGCAGGGGGTCGGAGTTGGAGTTGGGGTTTtAGGgTTtGGTGAGgAaGAGaGAGGAaaGGgAaGGgTTtGAaTtGGGgAAAAaaTGTtGGATTTTtCAGAGAGGGGGTGAAGAGTGGgAAGGAGATgAAGGTGGGTGGCAATGGCATTGTAGAGAGAGATTAGAGAGAGAGAGAGAgNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAACTCAATTCTTGAGGCCGCAAACaCAATTCATGAGGCCTCGAACTCAGACTTTGTGGCCGAAaTGCTTTGGCTAAAAAAaaaCATTTTtttCTTtGGTCTTCATTAAAATAAAAaTTTGCATtcGTTAGTTTTGCGACGATTTTTGTGGTCTATTGGTTCGTtCcGACGAGATGAATCTAAAgAGtaTAAAAttAAAATTTTtaaagaTTTCACTAAAGACAAAAaTAATCTAGATTTTTgTgATTtATTAGATTgTTTTtGTCTTTATTCaCAAAAATTTAAGTTtCGTTCATAATTTTAtACTTTTTtAGATTCCTCTCGtGgAGAGAATCCAATAAATCACAAAAAAaTCGATGCAAAACTAGCAAAAACAAATTtCTTTTGAaTTAAAAAAAAaCATTTtAGTTTTTTtAGCCAAAGCAAGTGAACGAAACTTGAATCTTGTGACCTGAAACTcccACCTTGTGACCACTAACTCGAACCTTTTGGCCTCAAACTCCAACCCTTtGGGCCGACAAAATGTtATTtCTCATCTTAAAAActtAGGCCTaTTTTGGCCAAAGTAGAAAATCAAGCTTTtCcTTTtAgCTTTTTTTTTTttGGgAAAAAAaCTCAAAGGTAAAaGGTGGATtGTTTtGGCcTTTTTTttGTGCTTTTTGCTTTTCACCTTTCAAATTGTAATtGTTTTTTtATtACTTTTtGCTTATtGTAAaGTTACTTCTTTAAGCCAAATTCTATGACTTTtCgAaTCtCAAAAAAGCAAAAGGCTTGCTTTTCTAGTTTGGCCAAAATAAGCcTTtATCTCCATAAtttgttGACTACTTTGTtATTCATGATTTTTAaTCTTTAGtgATTTCTGTCAAATATTTtATTTTTTCCCTTTTTAGATGCATTCATACCTCTgTTTtAAAAAATATAAAAAaaaTTATGATATTAAGAAAAAaGACTAAAAaCgAAAAaTAGGCAACTATATACAAGCCTTTTtAAtCtAaaTAAAAAaCTCATCCAAaTAAATGCTACTCTAACTTGTTTTtCTCAACACTTTTGTCAACAACGTTTAAaTAATCTTGTGAAATGGAAATGAAAACTATTTTAACGCTCCTTTTTTTCTTTCTATCTATTGgCACTCAATTTCTTATCTTTTATtgCGTGACAAAaCTAAACTATCCTTGgTtCTTATCTCTTGAGTCAATTTTTtCATATAAAAGGTAaTTCGAtACTtCCGCATGAATAAAAACCAAAAAaTGGGTGCCAATGGATAAAAAaTTaGCGTTAAAatCATTTCCCAAAATAAAaaTaGTTgCGTCAAGGTTCTAAAtaCCATAtCGTTTTTTCtAGTTGTACAATATGAACCAGtNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTGCACTATGCTGCACCCTCCTCagATATTTTTGGTCagCTTCCCGCCAAACCCACTAGgCTAAAACCCTAGTTGCCTTTTCCCCCACTCCAaAACCCTAGTTCtCTCTCTCTCCCTCCGCCACCAGTTCCCCACCCcACCACAACCAACCATGGACCCTCAGTCcGTTCAGACAAACCTAGTCCTCATCCTAGACTTCGGTtCTCAGTACACCCACCTCATCACgCGACGCATCCGATCCCTCTCCGTCTTCTCCCTCTGCATCTCAGGCACCTCCCCTCTCAAATCCATCACAGACCtAAaCCCAAGTGTCATAATCCTCTCCGGTGGTCCCCACTCGGTtCATgCGCCCAACGCGCCGTGTTTCCCtGATGGGTTCATAGAGTATGTGGAGTCTAAGGGCGTGTTTGTTTtGGGCATATGTTATGGGTTGCAGCTGATTGTGGAGAGGCTGGGTGGGTGTGTtGGGgTtGGCGAGAAGCAGGAGTATGGGAGGATGGAGATTGAGGTGGAGAAGGCTTGTGGTTTGTTTGGTGGGTCAAAGAAAGTTGGTGATAAGCAGGTTGTTTGGATGAGTCACGGGGATGAGGCTGTGAAGTTGCCTGATGGGTTTGAAGTGGTGGCGCGGAGTCAGCAGGGGtcGGTGGCGGCTGTGGAGTGTCCTGGGAGAAGGTTTTATGGCCTTCAGTATCATCCGGAGGTAATTGGGTATCTTCATTCATTTCATTCTTGGCCTTGTGATACGCTGCCCTGTTTTAATTGTCTTTGTAATTTGGTtCTTTGGCTGGCTTTGTTTGGTTCTTTACTAGTGGTTATTGTTTGTACGCGAAGTGCTTACTTGCTGATATCATCTTCATGTTGAAGTGGTAAtAATGAATGAAAAATGGAATGAATTGACCCAATATCTGAAAAGTTATCGTCTTTAGTGGTACTGTGGTAGTGGCCTATAAAACTGTTAGCGAcACTCCCTCGtGATGTATCCTGCTCAGGCCcAGATCCTTAGCAAGAATGAGTCAATGACCATTGATCCCTTTtCAAGCACTTAAGCtgAaCTTCCACATGgTACCATAAAATCCCTGAATCCTCTCAGCACAAGCACTGATGATCTCTCTGTTTGAGTTTCACATCACTAGCCCAACCCCCAGAAAACcATCCTTATAGTTATAGTAGCCCCGTCAAACTGAATTGTGAGCTCACCTTCATTTGGAGCTTTCCATGAGCCAGCAAGATGTTGGGATAGTACTACAGTCTTTGGAAATACAATGGGAGTTCTCTCAGGTAGCTAGCTGCATATTCtGCAAGATCACTGGCTCCCCTGTTTGTCCCtccAAAAAaTGTAATTGCCTATTTAACCAAATTACCCAAGCCACAATTAGAAagAGATCTATTATTtGTTGGAACCAACCCCCTCAATCTGCATAGCCCAGAGATGCCCCTTTCTTCAAGCACATGCAAATCTTTGAATCGATAACCTTCCGTAAcATTCTTTGCTTACTCACaCtCTTTTAGACAACGAATGATGGTCTCCGGTGCGGCGCTGTCCCTCCACACAGGCTACAACAATCCTTAATATTAACCATTTTTTtGACAAGCTTATGGTTCATTGGGAAAATCCCCAAACACAtCTTTCAcacaatgaCACAAACAATTTACTTTgTGTTGGgAaTTTTCAGATTCCAGATTTCTCCTAAGATCTcATATTATCCATGTCAGAAGATGAGAGTTGAGAGTgCTGAAAATGTAATGTCCCTTGTGGTGTAACCAGACtCcATGGTGTAAGTTTCTTGTTTAGTGAAGTTCCATGTTTAGTTGTCTTCATTTCCCAGGaTAATAGGGGAATGTTTCATGtCcTATgAGCTTCAAAAGGGaTAAAaGATCACAGCTAAGATGTTCCTTCCAAACCCCAGTATTGTGGTCAACGAGGCCCTTtACTaCCATTCTCTCCGTATCAGGCTTACAaGAGCTTGTAGTTTTAaGGGAGAAGTGCTTGGgATCCATtGGTCTGGCCACACCCTGGTTTtAtATCCcTTTCcGATCCtCcACCTAAGaCCCTTTTTAAGCACCTCCATACCCTCACACAT
>DR067752 Length = 574
Score = 46.1 bits (23), Expect = 3e-04 Identities = 29/31 (93%) Strand = Plus / Minus
Query: 459 gaagaactgatgccgaatagatgcaaatagg 489
||||||| ||||||||| |||||||||||||
Sbjct: 353 gaagaacagatgccgaacagatgcaaatagg 323
12) UFGT
search with amino acids using tBLASTn
SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGKEL
>CV091309 Length = 663
Score = 124 bits (310), Expect = 1e-30 Identities = 60/60 (100%), Positives = 60/60 (100%) Frame = +3
Query: 1 SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGKEL 60
SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGKEL
Sbjct: 138 SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGKEL 317
>DR067838 Length = 557
Score = 119 bits (297), Expect = 4e-29 Identities = 58/60 (96%), Positives = 58/60 (96%) Frame = +1
Query: 1 SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGKEL 60
SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKG L
Sbjct: 352 SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGTGL 531
