J-GGA Scaffold Sequences

J-GGA Scaffold Oligos

Prefix: Gaattcgcggccgcttctagag

Junction A: Atagtctgttcatggtgc

Spacer: acgt

Junction B: Tattgatgtagttaaggc

Spacer: cgga

Junction C: Tttctaatggggaccatc

Spacer: ttag

Junction D: Ttaagctatgtattcctg

Suffix: tactagtagcggccgctgcag

Top Strand

SCAFF_TOP1; AATTCGCGGCCGCTTCTAGAGATAGTCTGTTCATGGTGCACGTTATTGATGTAGTTAAGGCCGGATTTCTAATGG

SCAFF_TOP2; GGACCATCTTAGTTAAGCTATGTATTCCTGTACTAGTAGCGGCCGCTGCA

BOTTOM STRAND (5’ TO 3’)

SCAFF_BOT1; GCGGCCGCTACTAGTACAGGAATACATAGCTTAACTAAGATGGTCCCCATTAGAAATCCGGCCTTAACTAC

SCAFF_BOT2; ATCAATAACGTGCACCATGAACAGACTATCTCTAGAAGCGGCCGCG

Annealed AATTCGCGGCCGCTTCTAGAGATAGTCTGTTCATGGTGCACGTTATTGATGTAGTTAAGGCCGGATTTCTAATGGGGACCATCTTAGTTAAGCTATGTATTCCTGTACTAGTAGCGGCCGCTGCA

   GCGCCGGCGAAGATCTCTATCAGACAAGTACCACGTGCAATAACTACATCAATTCCGGCCTAAAGATTACCCCTGGTAGAATCAATTCGATACATAAGGACATGATCATCGCCGGCG

PRIMERS TO BE USED FOR INVERTED PCR OF SCAFFOLD