DM Notes 1.21.16
From GcatWiki
FastQC Reports: how to read
Illumina sequencing
Per base sequence quality: x-axis is bp. Quality score from 0 to 40 (40 being perfect score, 20 and higher is considered good). Whiskers could represent different things.
Per tile sequence quality: these were all read simultaneously. Put cDNA in a device about the size of palm. Since all were done through the same device, makes sense that everyone in the class has the same panel. Not sure whether red flag is a deal breaker.
Per sequence quality scores: most of the sequences have a high quality (peak at 39). Anything from 20 or higher is considered good, but there are a few reads that aren't very good (below 20).
Per base sequence content:
