Davidson Missouri W/Davidson Protocols

• What is the list of DNA parts that we will need for the first stage, second stage, entire project?
  
• ERIN and SABRIYA Which DNA parts exist in the registry?Answer1More Parts
  
• ERIN and SABRIYAWhich DNA parts will have to be designed? Will they be synthesized or produced by PCR?
  

loxP sites Hin/Hix CRE gene Degradation Tags

1. Common molecular reagents
2. Standard Assembly
3. Compatibility of Plasmids
4. Building dsDNA with Oligos
5. Setting up PCR mixtures
6. PCR and Mg²⁺ concentration
7. Clean and Concentrate DNA (after PCR, before digestion)
8. Pouring an agarose gel
9. Calculate MWs
10. Digest DNA with restriction enzymes
11. Double Digest Guide
12. Ethanol Precipitate DNA (short protocol)
13. 1kb MW markers
14. Shrimp Alkaline Phosphatase
15. Qiagen QIAquick Gel Purification
16. Qiagen QIAquick Column Regeneration Protocol
17. ElectroElute Gel Purification
18. Ligation Protocol
19. Heat Shock Transformation OR Short version of Heat Shock
20. Zippy Transformation
21. Colony PCR to Screen for Successful Ligations
22. Promega miniprep
23. Choices for Transformation: Heat Shock vs. Zyppy
24. Choices for Mini-Preps: Promega vs. Zyppy