Gel Electrophoresis for Bio113

Analyzing PCR Results Using Gel Electrophoresis

1. A 2.0% agarose gel has been poured for you. It contains 0.5X TBE buffer.
2. You will start with the tubes containing your PCR experiments. You should have some eXperimental tubes and one Negative control tube.
3. Take 20 µL of each green PCR solution and load each into its own well. Record the location of each sample you load.
4. Dr. C. will load 5 µL of the 1 kb molecular weight marker ([http://www.bio.davidson.edu/people/macampbell/113/weekly_Labs/1kbladder.png also called 1 kb ladder).
5. The gel contains Midori Green which is a dye that fluoresces green when it is bound to dsDNA.
6. The gel will run at about 100 volts for about 30 minutes. We will photograph the gel and you will get your own hard copy of the final results.