Construction of a genetic toggle switch in E. coli
Authors
Timothy Gardner
Charles Cantor
James Collins
Methods
Supplementary Material
Helpful Information
--If you are having trouble determining the meaning of Type I-IV plasmids, look at the first page of the supporting materials [1]. Each plasmid type has a different construction and purpose. Types I-III serve as control plasmids, while Type IV plasmids are the ones responsible for the toggle switch.
--Ribosomal Binding Site (RBS) efficiency refers to the likelyhood that a given RBS will attract a ribosome for translation of mRNA. In this paper, varying RBS strengths allowed for control of the rate of protein synthesis.
--Cooperativity of the repressor when binding to a promoter is an important aspect of the mathematical model presented in this paper. Click here for more information on cooperative binding.
