Wet Lab Pages

From GcatWiki
Revision as of 19:45, 26 May 2008 by AnGordon (talk | contribs) (Registry parts to clone)
Jump to: navigation, search

This is the space for MWSU and DC wet lab students to create content. Our part numbers will be in this range only: BBa_K091000 to BBa_K091999.

  1. Davidson Wet Lab Protocols
  2. MWSU Wet Lab Protocols
  3. Plasmid Compatability
  4. Davidson -80 Stocks
  5. Using Amp and Amp^R as communication
  6. FAQs for Wikis



Registry parts to clone

Composite Parts:

Part Description Student Responsible (DC or MW) Location and Vector Resistance Results from 5/22/08
BBa_F2621 AHL receiver with lux pR and codes for LuxR Karlesha Roland (DC) 1001 12B pSB1A2 amp growth MW
BBa_I15030 Lux-sender(Autoinducing)Codes for LuxI and LuxR Kelly Davis (DC) will this send to part BBa_I13263 1015 8F pSB3K3 kan growth MW
BBa_I13263 Lux Receiver (HSL & R0063 driven)produces YFP Pallavi Penumetcha (DC) 1002 8E pSB1A2 amp growth MW & DC
BBa_f2622 3OC6HSL receiver that outputs PoPS James Barron (DC) 1001 12E pSB1A2 amp no growth
BBa_F1610 Device that receives PoPS and outputs LuxI Malcolm Campbell (DC) 1016 10D pSB1AK3 amp & kan growth MW
BBa_I0426 Las-reciver(EYFP) Kristi Muscalino (DC) 1008 5B pSB1A2 amp growth MW & DC
BBa_I0407 LasI test Erin Feeney (DC) 1008 4F pSB1A2 amp growth MW & DC
BBa_I0466 RhlR Protein Generator without LVA Laurie Heyer (DC) 1001 5C pSB1A2 amp growth MW

Basic Parts:

Part Description Student Responsible (DC or MW) Location and Vector Resistance Results from 5/22/08
BBa_C0061 LuxI gene with LVA Madeline Parra (DC) 1000 3E pSB1A2 amp growth MW
BBa_C0161 LuxI gene without LVA Xiao Zhu (MW) 1001 8B pSB1A2 amp
BBa_C0062 LuxR gene without LVA John Igo (MW) 1000 3F pSB1A2 amp
BBa_R0063 lux pL Aaron Lewis (MW) 1000 4H pSB1A2 amp
BBa_R0062 lux pR Andrew Gordon(MW) 1000 4G pSB1A2 amp
BBa_C0070 rhlI gene Jeff Poet (MW) 1013 3E pSB2K3 kan
BBa_C0071 rhlR gene with LVA Max Win (DC) 1013 3F pSB2K3 kan no growth
BBa_C0171 rhlR gene without LVA MWSU 1001 6F pSB1A2 amp
BBa_R0079 las pR Todd Eckdahl (MW) 1001 10F pSB1A2 amp
BBa_C0079 lasR gene Robert Cool (MW) 1013 4F pSB2K3 kan
BBa_C0078 LasI gene Alicia Allen (MW) 1013 4E pSB2K3 kan
BBa_J07019 fecA promoter with Fur box Not in Registry
BBa_#### Description Sven(MW)
BBa_#### Description Sven(MW)
BBa_#### Description Sven(MW)

Fluorescent Protein Gradient Testers:

Part Description Student Responsible (DC or MW) Location and Vector Resistance Results
BBa_J04450 Lac-RBS-RFP-T Robert Cool (MW) 1004 4f pSB1A2 amp
BBa_J04451 pLac-RBS-RFP w/ LVA tag-T-T Erin Feeney and Kelly Davis (DC) 1016 9F pSB1AK3 amp and kan
BBa_I13520 pBAD-RBS-mRFP-T-T Erin Feeney and Kelly Davis (DC) 1009 7B pSB1A2 amp
BBa_J5528 pBAD-RBS-GFP-T-T Erin Feeney and Kelly Davis (DC) 1015 7A pSB2K3 kan
BBa_J04430 pLac-RBS-GFP-T-T Erin Feeney and Kelly Davis (DC) 1004 4H pSB1A2 amp


Parts for Sending and Receiving Signals:

Part Description Student Responsible (DC or MW) Location and Vector Resistance Results
BBa_J07019 fec A promoter with fur box James Barron (DC) Not in Registry-Needs to be amplified
BBa_F2622 3OC6HSL Receiver Device Pallavi Penumetcha (DC) 1001 12e pSB1A2 amp
BBa_I14032 pLac promoter Pallavi Penumetcha (DC) 1013 8e pSB2K3 kan
BBa_S03632 lacI promoter and luxI gene Pallavi Penumetcha (DC) 1016 7f pSB1AK3 amp and kan
BBa_E0240 RBS and GFP Pallavi Penumetcha (DC) 1001 4B pSB1A2 amp