Designing XOR Gates - two campus approach

Davidson Approach

Here is an idea Malcolm had.

Please look at this and ask questions and find holes in it now so we don't waste time building something that won't work.

XOR Biological Design

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The idea is to have two mirrored halves of the system. One is regulated by AI-1 and the other by AI-2. There is a potential problem in that the Lux half is more likely to get positive feedback than the Las half. This MAY not be a problem because 0/0 is leaky so we put a weak RBS to minimize leaky protein production. Also, if we add AI-2 and AI-1 is produced by leak, then the entire system shuts down. The repressor site is located between -35 and -10 of the promoter. The activator binding site is upstream of -35. This has been documented by Egland and Greenberg

Ampicillin Communication: time delayed cell growth

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Growth Layouts

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MWSU Approach