LasR/cI

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Goal:

pBAD+RBS+lasR+TT(James DC)

S0388?(I13453+B0034+C0079+B0015)

pLasR/cI+RBS+LuxI+RBS+Mnt+TT

K091141+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015

Ligation Plan

Vector Insert Product
Step BBa_ Description Pre-/suffix Enzymes Size BBa_ Description Pre-/suffix Enzymes Size BBa_ Size
1 K091141 RBS+Mnt+TT R/X;S/P R/X 468+2079 C0261 RBS+luxI R/X;S/P R/X 661 TBD
2 K09114 plasR/cI R/X;S/P S/P 164+2079 TBD RBS+luxI+RBS+Mnt+TT R/X; S/P X/P TBD

Parts

BBa_ Description Source location('07) Vector Responsible
B0015 double terminator registry 07 Plt.1-1I;Plt.3-3O pSB1AK3
I13025 RBS+Mnt registry 07 Plt.1-23J pSB1A2
K091141 plasR/cI MW N/A pSB1A2
C0261 RBS+luxI registry 07 Plt.1-24A pSB1A2