Difference between revisions of "Bacterial Media"
| Line 21: | Line 21: | ||
'''Plate Codes''' | '''Plate Codes''' | ||
| − | + | #Black line only LB none | |
| − | #Black only LB none | + | #Black line, Red line LB Amp |
| − | #Black, Red | + | #Black line, Blue line LB Tet |
| − | #Black, Blue LB Tet | + | #Black line, Green line LB Kan |
| − | #Black, Green LB Kan | + | #Black line, Cyan line LB Chlor |
| − | #Black, Cyan | ||
'''Ampicillin''' | '''Ampicillin''' | ||
Revision as of 14:33, 31 May 2010
Missouri Western Synthetic Biology Protocols Bacterial Media Prep
LB Broth
5 g Yeast Extract 10 g Tryptone 5 g NaCl
QSV 1 liter
LB Plates (makes about 35 plates)
5 g Yeast Extract 10 g Tryptone 5 g NaCl 15 g BactoAgar
QSV 1 liter
Plate Codes
- Black line only LB none
- Black line, Red line LB Amp
- Black line, Blue line LB Tet
- Black line, Green line LB Kan
- Black line, Cyan line LB Chlor
Ampicillin
Stock – 50 mg/ml
Weigh out 2.5 g Ampicillin, sodium salt (Sigma A0166) Dissolve in 25 ml dH20 Add 25 ml Ethanol
Working concentration 50 ug/ml (1 ml stock solution per liter)
Tetracycline
Stock – 5 mg/ml
Weigh out 0.25 g Tetracycline HCl (Sigma T7660) Dissolve in 50 ml 70% Ethanol Mix/vortex vigorously
Working concentration 20 ug/ml (4 ml stock solution per liter)
Kanamycin
Stock – 10 mg/ml
Weigh out 0.5 g Kanamycin sulfate (Sigma K1377) Dissolve in 50 ml dH20
Working concentration 20 ug/ml (2 ml stock solution per liter)
Chloramphenicol
Stock – 25 mg/ml
Weigh out 1.25 g Chloramphenicol (Sigma C0378) Add 50 ml 100% Ethanol Mix/vortex vigorously
Working concentration 25 ug/ml (1 ml stock solution per liter)
