Difference between revisions of "Common Restriction Sites"
From GcatWiki
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| − | '''These restriction sites are all optimal at 37 degrees Celsius and are organized by the kind of overhang they create. | + | '''These restriction sites are all optimal at 37 degrees Celsius and are organized by the kind of overhang they create. *denotes it is affected by methylation (CpG, dcm, or dam)''' |
| − | *denotes it is affected by methylation (CpG, dcm, or dam)''' | ||
---- | ---- | ||
| Line 56: | Line 55: | ||
* XhoI * | * XhoI * | ||
* XmaI * | * XmaI * | ||
| + | |||
| + | '''Blunt cutters:''' | ||
| + | * AfeI * | ||
| + | * BsrBI* | ||
| + | * DraI | ||
| + | * EcoRV * | ||
| + | * FspI * | ||
| + | * HpaI * | ||
| + | * MscI * | ||
| + | * NaeI * | ||
| + | * NruI-HF * | ||
| + | * PmeI * | ||
| + | * PmlI * | ||
| + | * PvuII-HF | ||
| + | * ScaI-HF | ||
| + | * SfoI * | ||
| + | * SnaBI * | ||
| + | * SspI-HF | ||
| + | * StuI * | ||
Latest revision as of 23:59, 27 September 2018
These restriction sites are all optimal at 37 degrees Celsius and are organized by the kind of overhang they create. *denotes it is affected by methylation (CpG, dcm, or dam)
3’ Overhang:
2 Sticky Ends-
- AsiSI *
- PacI
- PvuI-HF *
- SacII *
4 Sticky Ends-
- AatII *
- BmtI/BmtI-HF
- FseI *
- KpnI-HF
- NsiI-HF
- PstI-HF
- SacI-HF *
- SbfI/SbfI-HF
- SphI-HF
5’ Overhang:
2 Sticky Ends-
- AcII: *
- ClaI *
- NarI *
- NdeI
4 Sticky Ends-
- AflII
- AgeI-HF
- ApaLI *
- AscI *
- AvrII
- BamHI-HF
- BclI-HF *
- BsaI-HF V2 *
- BspHI *
- BsrGI-HF
- EagI-HF *
- EcoRI-HF *
- HindIII-HF
- KasI *
- MfeI/MfeI-HF
- MluI-HF *
- NcoI-HF
- NgoMIV *
- NheI-HF 4SE *
- NotI-HF *
- PspOMI *
- SalI-HF *
- SpeI/SpeI-HF
- XbaI *
- XhoI *
- XmaI *
Blunt cutters:
- AfeI *
- BsrBI*
- DraI
- EcoRV *
- FspI *
- HpaI *
- MscI *
- NaeI *
- NruI-HF *
- PmeI *
- PmlI *
- PvuII-HF
- ScaI-HF
- SfoI *
- SnaBI *
- SspI-HF
- StuI *
