Common Restriction Sites

3’ Overhang: 2 Sticky Ends-

• AsiSI *
• PacI
• PvuI-HF *
• SacII *

4 Sticky Ends-

• AatII *

• BmtI/BmtI-HF • FseI * • KpnI-HF • NsiI-HF • PstI-HF • SacI-HF * • SbfI/SbfI-HF • SphI-HF