Difference between revisions of "Construction of a genetic toggle switch in E. coli"
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==Helpful Information== | ==Helpful Information== | ||
| − | If you are having trouble determining the meaning of Type I-IV plasmids, look at the first page of the supporting materials [http://www.bio.davidson.edu/courses/synthetic/papers/Collins_SOM.pdf]. Each plasmid type has a different construction and purpose. Types I-III serve as control plasmids, while Type IV plasmids are the ones responsible for the toggle switch. <br> | + | --If you are having trouble determining the meaning of Type I-IV plasmids, look at the first page of the supporting materials [http://www.bio.davidson.edu/courses/synthetic/papers/Collins_SOM.pdf]. Each plasmid type has a different construction and purpose. Types I-III serve as control plasmids, while Type IV plasmids are the ones responsible for the toggle switch. <br> |
| − | Ribosomal Binding Site (RBS) efficiency refers to the likelyhood that a given RBS will attract a ribosome for translation of mRNA. In this paper, varying RBS strengths allowed for control of the rate of protein synthesis. <br> | + | --Ribosomal Binding Site (RBS) efficiency refers to the likelyhood that a given RBS will attract a ribosome for translation of mRNA. In this paper, varying RBS strengths allowed for control of the rate of protein synthesis. <br> |
| − | [http://en.wikipedia.org/wiki/Bistable What does bistable mean?] | + | --[http://en.wikipedia.org/wiki/Bistable What does bistable mean?] |
| − | Cooperativity of the repressor when binding to a promoter is an important aspect of the mathematical model presented in this paper. [http://en.wikipedia.org/wiki/Cooperativity Click here] for more information on cooperative binding. | + | --Cooperativity of the repressor when binding to a promoter is an important aspect of the mathematical model presented in this paper. [http://en.wikipedia.org/wiki/Cooperativity Click here] for more information on cooperative binding. |
| − | [http://en.wikipedia.org/wiki/Bimodal What is a bimodal distribution?] | + | --[http://en.wikipedia.org/wiki/Bimodal What is a bimodal distribution?] |
| − | [[Will DeLoache - Synthetic Biology Seminar | Back to Main Page]] | + | --[[Will DeLoache - Synthetic Biology Seminar | Back to Main Page]] |
Revision as of 19:08, 5 October 2007
Authors
Timothy Gardner
Charles Cantor
James Collins
Methods
Supplementary Material
Helpful Information
--If you are having trouble determining the meaning of Type I-IV plasmids, look at the first page of the supporting materials [1]. Each plasmid type has a different construction and purpose. Types I-III serve as control plasmids, while Type IV plasmids are the ones responsible for the toggle switch.
--Ribosomal Binding Site (RBS) efficiency refers to the likelyhood that a given RBS will attract a ribosome for translation of mRNA. In this paper, varying RBS strengths allowed for control of the rate of protein synthesis.
--Cooperativity of the repressor when binding to a promoter is an important aspect of the mathematical model presented in this paper. Click here for more information on cooperative binding.
