Correlative Data
HB Fraser et. al. used a correlative approach to study the evolution of gene noise. They used the assumption that lower translational efficiency results in lower stochasticity in the production of the gene. They then estimated protein production rates for all yeast genes. They then experimentally measured mRNA levels and compared the estimated protein production level with the expressed mRNA level. Using a bin array they found that genes deemed essential and genes participating in the formation of large complexes were low in translational efficieny (they used a spearman partial test to determine whether their data was statistically significant). They hypothesize that the minimization of noise in a cell is expensive. Expense comes from need to continually synthesize and degrade mRNA transcripts is energetically unfavorable. Tight control over stochasticity is only selected for when the cost of wasting an entire protein complex or cell death outweighs the cost of minimizing stochastic translation.
