Difference between revisions of "Freeze glycerol stocks of E. coli"
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Latest revision as of 20:41, 9 August 2022
To preserve all stocks of each strain derived from a unique colony, we will freeze glycerol stock at -80 C.
- Put 37.5 µL of warmed glycerol in a pre-labeled sterile 1.5 mL tube.
- Remove 212.5 µL of your overnight culture and add it to the appropriately labeled tube with glycerol.
- Close the lid tightly and mix by vortexing.
- When all 4 tubes are well mixed, give them to your instructor to put in the freezer.
